反相高效液相色谱梯度洗脱法测定糖维胶囊中盐酸小檗碱和葛根素的含量

[Determination of beberine hydrochloride and puerarin in tangwei capsules by RP-HPLC of gradient elution method].

作者信息

Wang Y J, Yang X J, Zhang Y H, Zhao W G

机构信息

Changchun Municipal Institute for Drug Control, Changchun 130061, China.

出版信息

Se Pu. 2000 Sep;18(5):465-7.

PMID:12541715

Abstract

A reversed-phase high performance liquid chromatographic gradient elution method was developed for the determination of beberine hydrochloride and puerarin in Tangwei capsules. Separation was obtained by using Hypersil ODS (150 mm x 4.6 mm i.d., 5 microns) column and mobile phase of methanol and acetic acid, triethylamine water solution. Detection was performed with UV detector at 230 nm. The column temperature was 35 degrees C. The calibration curves were linear within the range of 0.18 microgram-1.66 micrograms for beberine hydrochloride (n = 5, r = 0.9999) and 0.09 microgram-0.89 microgram for puerarin (n = 5, r = 0.9998). The recovery and RSD of beberine hydrochloride were 98.2% and 1.6% and those of puerarin were 97.5% and 1.8%, respectively.

摘要

建立了一种反相高效液相色谱梯度洗脱法，用于测定糖胃胶囊中盐酸小檗碱和葛根素的含量。采用Hypersil ODS柱（150 mm×4.6 mm内径，5微米），以甲醇和醋酸、三乙胺水溶液为流动相进行分离。用紫外检测器在230 nm波长处进行检测。柱温为35℃。盐酸小檗碱在0.18微克-1.66微克范围内校准曲线呈线性（n = 5，r = 0.9999），葛根素在0.09微克-0.89微克范围内校准曲线呈线性（n = 5，r = 0.9998）。盐酸小檗碱的回收率和相对标准偏差分别为98.2%和1.6%，葛根素的回收率和相对标准偏差分别为97.5%和1.8%。