高效液相色谱法测定心脉通口服液中葛根素的含量
[Determination of puerarin in xinmaitong oral liquid by HPLC].
作者信息
Huang Y F, Zhang Y M, He A, Cao X H
机构信息
Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, Guangdong, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2001 Nov;26(11):760-1.
OBJECTIVE
To develop a method to determine the content of puerarin in Xinmaitong oral liquid.
METHODS
HPLC method was used. The separation was performed on Suntek kromasil C18 column with methanol-water containing 0.5% acetic acid (25:75) as a mobile phase and the wavelength of UV detector was 250 nm.
RESULTS
The linearity of puerarin was good and average recovery was 97.8%.
CONCLUSION
The method is simple, reliable and sensitive. It also shows good resolation. It can be used in quality control of Xinmaitong oral liquid.
目的
建立一种测定心脉通口服液中葛根素含量的方法。
方法
采用高效液相色谱法。分离在Suntek kromasil C18柱上进行，以含0.5%乙酸的甲醇-水（25:75）为流动相，紫外检测器波长为250nm。
结果
葛根素线性关系良好，平均回收率为97.8%。
结论
该方法简便、可靠、灵敏，分离度好，可用于心脉通口服液的质量控制。
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