Feely J, Kavanagh P V, McNamara S M
Department of Pharmacology and Therapeutics, Trinity College, Dublin.
Ir J Med Sci. 1999 Jul-Sep;168(3):189-92. doi: 10.1007/BF02945851.
A sensitive gas chromatography/mass spectrometry method for the detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, is described. The method is based upon solvent extraction followed by derivatisation with methyl-bis-trifluoroacetamide (MBTFA), to give a trifluoroacetyl derivative. A profile of 7-aminoflunitrazepam urinary concentrations following ingestion of 0.5 to 4 mg oral doses is reported. The method has been found to be reproducible and can be used for the confirmation of flunitrazepam administration.
本文描述了一种用于检测和定量氟硝西泮主要尿液代谢物7-氨基氟硝西泮的灵敏气相色谱/质谱法。该方法基于溶剂萃取，然后用甲基双三氟乙酰胺（MBTFA）进行衍生化，以得到三氟乙酰衍生物。报告了摄入0.5至4毫克口服剂量后7-氨基氟硝西泮的尿液浓度情况。已发现该方法具有可重复性，可用于确认氟硝西泮的服用情况。
