血小板因子刺激在血浆血清中处于静止状态的成纤维细胞和平滑肌细胞增殖。
Platelet factors stimulate fibroblasts and smooth muscle cells quiescent in plasma serum to proliferate.
作者信息
Rutherford R B, Ross R
出版信息
J Cell Biol. 1976 Apr;69(1):196-203. doi: 10.1083/jcb.69.1.196.
Abstract
Whole blood serum is widely recognized as essential for the growth of diploid cells in culture. Dermal fibroblasts and arterial smooth muscle cells fail to proliferate in culture in the presence of serum derived from platelet-poor plasma. Platelet-poor plasma serum is capable of maintaining monkey arterial smooth muscle cells quiescent in culture at either low (1.5 x 10(3)) or high (2.0 x 10(4)) population densities. The proportion of cell traversing the cell cycle under these conditions was approximately 3%. Equal numbers of quiescent smooth muscle cells initiated DNA synthesis and cell division when treated with whole blood serum or with an equivalent quantity of platelet-poor plasma serum supplemented with a factor(s) derived from a supernate obtained after exposure of human platelets to purified thrombin in vitro.
摘要
全血血清被广泛认为是培养二倍体细胞所必需的。在缺乏血小板血浆来源的血清存在的情况下，真皮成纤维细胞和动脉平滑肌细胞在培养中无法增殖。缺乏血小板血浆血清能够使猴动脉平滑肌细胞在低（1.5×10³）或高（2.0×10⁴）细胞密度下在培养中保持静止。在这些条件下穿过细胞周期的细胞比例约为3%。当用全血血清或用等量的缺乏血小板血浆血清并补充来自人血小板在体外暴露于纯化凝血酶后获得的上清液中的一种或多种因子处理时，等量的静止平滑肌细胞开始DNA合成和细胞分裂。
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