3T3细胞中DNA合成起始的调控：低分子量营养素
Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.
作者信息
Holley R W, Kiernan J A
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2942-5. doi: 10.1073/pnas.71.8.2942.
Abstract
Sparse 3T3 cells, in excess serum, become quiescent in the early G(1) (or G(0)) phase of the cell cycle if the cells are cultured in low concentrations of amino acids, glucose, or phosphate ion. These quiescent cells then initiate DNA synthesis if the concentration of the limiting nutrient is increased. In normal medium, DNA synthesis in the same 3T3 cell line is controlled by serum factors. The results demonstrate the complexity of the control by external agents of DNA synthesis in these "normal," density-dependent cells.
摘要
在过量血清中，如果在低浓度氨基酸、葡萄糖或磷酸离子的条件下培养，稀疏的3T3细胞会在细胞周期的早期G(1)（或G(0)）期进入静止状态。如果限制营养物质的浓度增加，这些静止细胞就会启动DNA合成。在正常培养基中，同一3T3细胞系中的DNA合成受血清因子控制。这些结果表明，在这些“正常的”、密度依赖性细胞中，外部因素对DNA合成的控制具有复杂性。
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