Hosoya Akihiro, Yoshiba Kunihiko, Yoshiba Nagako, Hoshi Kazuto, Iwaku Masaaki, Ozawa Hidehiro
Division of Cariology, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho, 951-8514 Niigata, Japan.
Histochem Cell Biol. 2003 Jan;119(1):27-35. doi: 10.1007/s00418-002-0478-z. Epub 2002 Nov 27.
While dental pulp undergoes calcification following tooth replantation or transplantation, we actually know little about these mechanisms. We therefore conducted histological and immunohistochemical evaluations of mineralized tissue that formed in the pulp of rat maxillary molar transplanted into abdominal subcutaneous tissue. One, 2, 3, and 4 weeks post-transplantation, the teeth were investigated immunohistochemically using antibodies to osteocalcin (OCN), osteopontin (OPN), bone sialoprotein (BSP), dentin sialoprotein (DSP), and tissue non-specific alkaline phosphatase (TNAP). In the 1st week after transplantation, cell-rich hard tissue was formed at the root apex. At 2 weeks, formations of hard tissue, with few cells in the root canals and bone-like tissue in the coronal pulp chamber, were noted. After 3 and 4 weeks, the amounts of these hard tissues were increased. The immunolocalization of OCN, OPN, and BSP was seen strongly in coronal and apical hard tissues, but weakly in the root hard tissue. Conversely, DSP localized in the root hard tissue, but not in other newly formed hard tissues. At 1 week, TNAP localized along the periphery of the apical hard tissue and the lower surfaces of root predentin. These results demonstrate that the newly formed hard tissues in the pulp cavity of subcutaneously transplanted molars could be classified into three types, suggesting that these might be formed by type-specific cells.
虽然牙髓在牙齿再植或移植后会发生钙化，但我们实际上对这些机制知之甚少。因此，我们对移植到腹部皮下组织的大鼠上颌磨牙牙髓中形成的矿化组织进行了组织学和免疫组织化学评估。在移植后1、2、3和4周，使用抗骨钙素（OCN）、骨桥蛋白（OPN）、骨涎蛋白（BSP）、牙本质涎蛋白（DSP）和组织非特异性碱性磷酸酶（TNAP）的抗体对牙齿进行免疫组织化学研究。移植后第1周，根尖处形成富含细胞的硬组织。2周时，观察到根管内硬组织形成，牙髓冠部腔隙内有少量细胞和类骨组织。3周和4周后，这些硬组织的数量增加。OCN、OPN和BSP在冠部和根尖硬组织中免疫定位强烈，但在根部硬组织中较弱。相反，DSP定位于根部硬组织，而不在其他新形成的硬组织中。1周时，TNAP定位于根尖硬组织周边和根前期牙本质的下表面。这些结果表明，皮下移植磨牙牙髓腔中新形成的硬组织可分为三种类型，提示它们可能由特定类型的细胞形成。
