Wang Fengqin, Zhang Yongfa, Liu Jie, Song Andong, Liu Quanjun, Chen Wenxin
College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
Wei Sheng Wu Xue Bao. 2008 Jan;48(1):1-7.
Multilocus house-keeping gene sequence analysis is a hotspot of taxonomy and phylogeny of prokaryotes. In this research, we used atpD and gln II gene sequences to analyze the phylogeny of nine rhizobia strains of Albizia spp., Acacia spp. and Leucaena leucocephala and compared the results to that of 16S rDNA. The phylogenetic relationships based on the sequence analysis of these three genes were congruent at the genera level. CCBAU43060 and CCBAU 61139 were located in the branch of Rhizobium-Agrobacterium. CCBAU51471, CCBAU35220, CCBAU51276 and CCBAU61158 belonged to the genera of Mesorhizobium. CCBAU35234, CCBAU61178 and CCBAU35085 were assigned to Bradyrhizobium. Differences were found for some strains, for example CCBAU 61158, CCBAU43060, CCBAU61178, at the species level. Insertion fragment and mosaic gene were also found in some isolates. These results indicated that there was recombination between species in the same genera. It is reliable to determine the taxonomy status at genera levels based on the sequence analysis of 16S rDNA. If the relationships between strains belonging to the same genera were studied using the phylogeny methods, researches should be carried out with more than one house-keeping genes.
多位点持家基因序列分析是原核生物分类学和系统发育学的一个热点。在本研究中，我们使用atpD和gln II基因序列分析了合欢属、金合欢属和银合欢的9株根瘤菌菌株的系统发育，并将结果与16S rDNA的结果进行了比较。基于这三个基因序列分析的系统发育关系在属水平上是一致的。CCBAU43060和CCBAU 61139位于根瘤菌-土壤杆菌分支中。CCBAU51471、CCBAU35220、CCBAU51276和CCBAU61158属于中慢生根瘤菌属。CCBAU35234、CCBAU61178和CCBAU35085被归为慢生根瘤菌属。在一些菌株中发现了差异，例如CCBAU 61158、CCBAU43060、CCBAU61178在种水平上。在一些分离物中还发现了插入片段和镶嵌基因。这些结果表明同一属内的物种之间存在重组。基于16S rDNA序列分析确定属水平的分类地位是可靠的。如果使用系统发育方法研究同一属内菌株之间的关系，则应使用多个持家基因进行研究。
