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本文引用的文献

1
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.登革病毒的结构：对黄病毒的组装、成熟及融合的影响
Cell. 2002 Mar 8;108(5):717-25. doi: 10.1016/s0092-8674(02)00660-8.
2
Molecular and structural basis of the specificity of a neutralizing acetylcholine receptor-mimicking antibody, using combined mutational and molecular modeling analyses.运用联合突变分析和分子建模分析，解析模拟乙酰胆碱受体的中和抗体特异性的分子与结构基础。
J Biol Chem. 2000 Jul 14;275(28):21578-86. doi: 10.1074/jbc.M001794200.
3
Binding of an antibody mimetic of the human low density lipoprotein receptor to apolipoprotein E is governed through electrostatic forces. Studies using site-directed mutagenesis and molecular modeling.人低密度脂蛋白受体抗体模拟物与载脂蛋白E的结合受静电力支配。采用定点诱变和分子建模的研究。
J Biol Chem. 2000 Mar 10;275(10):7109-16. doi: 10.1074/jbc.275.10.7109.
4
Characterization of neutralizing anti-pre-S1 and anti-pre-S2 (HBV) monoclonal antibodies and their fragments.中和性抗前S1和抗前S2（乙肝病毒）单克隆抗体及其片段的特性分析
Mol Immunol. 1999 Jul;36(10):669-83. doi: 10.1016/s0161-5890(99)00074-7.
5
The atomic structure of protein-protein recognition sites.蛋白质-蛋白质识别位点的原子结构。
J Mol Biol. 1999 Feb 5;285(5):2177-98. doi: 10.1006/jmbi.1998.2439.
6
Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica.登革2型病毒包膜糖蛋白的单克隆抗体定位，牙买加。
Virology. 1998 Jul 5;246(2):317-28. doi: 10.1006/viro.1998.9200.
7
Japanese encephalitis virus antigenic variants with characteristic differences in neutralization resistance and mouse virulence.具有中和抗性和小鼠毒力特征差异的日本脑炎病毒抗原变异体。
Virus Res. 1997 Oct;51(2):173-81. doi: 10.1016/s0168-1702(97)00098-1.
8
Conformations of the third hypervariable region in the VH domain of immunoglobulins.免疫球蛋白重链可变区中第三个高变区的构象
J Mol Biol. 1998 Jan 16;275(2):269-94. doi: 10.1006/jmbi.1997.1442.
9
Site-specific mutagenesis of a recombinant anti-single-stranded DNA Fab. Role of heavy chain complementarity-determining region 3 residues in antigen interaction.重组抗单链DNA Fab的位点特异性诱变。重链互补决定区3残基在抗原相互作用中的作用。
J Biol Chem. 1997 Oct 24;272(43):26864-70. doi: 10.1074/jbc.272.43.26864.
10
Secondary structure of the 3'-noncoding region of flavivirus genomes: comparative analysis of base pairing probabilities.黄病毒基因组3'非编码区的二级结构：碱基配对概率的比较分析
RNA. 1997 Jul;3(7):779-91.

日本脑炎病毒包膜蛋白结构域III上的一个功能性表位决定簇与中和抗体结合位点相互作用。

A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with neutralizing-antibody combining sites.

作者信息

Lin Cheng-Wen, Wu Suh-Chin

机构信息

Institute of Biotechnology and Department of Life Science, National Tsing-Hua University, Hsinchu 30013, Taiwan.

出版信息

J Virol. 2003 Feb;77(4):2600-6. doi: 10.1128/jvi.77.4.2600-2606.2003.

DOI:10.1128/jvi.77.4.2600-2606.2003
PMID:12551998
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC141121/
Abstract

The envelope (E) protein of Japanese encephalitis virus (JEV) is associated with viral binding to cellular receptors, membrane fusion, and the induction of protective neutralizing-antibody responses in hosts. Most previous studies have not provided detailed molecular information about the spatial configuration of the functional epitopes on domain III of the E protein. Here site-directed mutagenesis was performed to demonstrate that the functional epitope determinants at Ser331 and Asp332 on domain III of the JEV E protein interacted with neutralizing monoclonal antibody (MAb) E3.3. Bacterial expression of the recombinant Fab E3.3 confirmed the molecular interactions of Arg94 in complementary determining region H3 with Ser331 and Asp332 on domain III. This study elucidates the detailed molecular structures of the neutralizing epitope determinants on JEV domain III, which can provide useful information for designing new vaccines.

摘要

日本脑炎病毒（JEV）的包膜（E）蛋白与病毒与细胞受体的结合、膜融合以及宿主中保护性中和抗体反应的诱导有关。以前的大多数研究都没有提供关于E蛋白结构域III上功能表位空间构型的详细分子信息。在此进行了定点诱变，以证明JEV E蛋白结构域III上Ser331和Asp332处的功能表位决定簇与中和单克隆抗体（MAb）E3.3相互作用。重组Fab E3.3的细菌表达证实了互补决定区H3中的Arg94与结构域III上的Ser331和Asp332之间的分子相互作用。本研究阐明了JEV结构域III上中和表位决定簇的详细分子结构，可为新型疫苗的设计提供有用信息。