State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Shanghai Veterinary Research Institute, Chinese Academy at Agricultural Sciences, Shanghai 200241, China.
Genes (Basel). 2023 Jun 22;14(7):1316. doi: 10.3390/genes14071316.
The Duck Tembusu virus (DTMUV), a pathogenic flavivirus, has been causing significant economic losses in the Chinese poultry industry since 2010. This virus can severely decrease egg production and inhibit the growth of laying ducks and ducklings. While many vaccines have been developed to prevent DTMUV infection, fresh outbreaks continue to occur, as few effective vaccines are available. The E glycoprotein of DTMUV is the primary target for inducing protective immunity in the natural host. Therefore, we conducted an investigation and successfully developed a recombinant baculovirus containing the DTMUV E gene. Ducklings were then vaccinated with the purified protein derived from this virus as a potential vaccine candidate. Our findings demonstrated that the E glycoprotein of DTMUV was highly expressed in Sf9 cells. The vaccination of ducklings with the recombinant baculovirus Bac-E resulted in the induction of strong humoral and cellular immune responses. Most significantly, we observed that the vaccine provided 100% protective immunity against lethal challenges with the DTMUV YY5 strain.
鸭坦布苏病毒（DTMUV）是一种致病性黄病毒，自 2010 年以来一直给中国家禽业造成重大经济损失。该病毒可严重降低产蛋量，并抑制产蛋鸭和雏鸭的生长。虽然已经开发了许多疫苗来预防 DTMUV 感染，但由于有效的疫苗很少，新的爆发仍在继续。DTMUV 的 E 糖蛋白是在天然宿主中诱导保护性免疫的主要靶标。因此，我们进行了一项调查并成功开发了一种含有 DTMUV E 基因的重组杆状病毒。然后，用源自该病毒的纯化蛋白对雏鸭进行了疫苗接种，作为一种潜在的疫苗候选物。我们的研究结果表明，DTMUV 的 E 糖蛋白在 Sf9 细胞中得到了高度表达。用重组杆状病毒 Bac-E 对雏鸭进行疫苗接种可诱导强烈的体液和细胞免疫应答。最重要的是，我们观察到该疫苗对 DTMUV YY5 株的致死性挑战提供了 100%的保护免疫力。
