Wang Y T, Liu W, Seah J N, Lam C S, Xiang J H, Korzh V, Kwang J
Institute of Molecular Agrobiology, The National University of Singapore, Singapore 117604.
Dis Aquat Organ. 2002 Dec 10;52(3):249-59. doi: 10.3354/dao052249.
White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes--GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.
通过聚合酶链反应（PCR）在墨吉对虾的血细胞、血淋巴和血浆中特异性检测到白斑综合征病毒（WSSV）。这表明虾血淋巴与该病毒之间存在密切关联。使用流式细胞术从虾中分离出三种类型的血细胞。观察了感染后不同时间墨吉对虾血细胞亚群的动态变化，表明WSSV感染选择性地影响特定亚群。免疫荧光分析（IFA）以及血细胞类型的瑞氏-吉姆萨双重染色研究进一步证实了病毒在受感染血细胞中的细胞定位。电子显微镜显示，在半颗粒细胞（SGC）的液泡和细胞核以及颗粒细胞（GC）的液泡中均发现了病毒颗粒。然而，在透明细胞（HC）中未检测到病毒。我们的结果表明，该病毒感染两种类型的虾血细胞——GC和SGC。SGC类型含有更高的病毒载量且表现出更快的感染率，显然更易受到WSSV感染。
