Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Fish Shellfish Immunol. 2011 Dec;31(6):1179-85. doi: 10.1016/j.fsi.2011.10.013. Epub 2011 Oct 20.
A Kazal type serine proteinase SPIPm2 is abundantly expressed in the hemocytes and shown to be involved in innate immune response against white spot syndrome virus (WSSV) in Penaeus monodon. The SPIPm2 is expressed and stored in the granules in the cytoplasm of semigranular and granular but not the hyaline hemocytes. Upon WSSV challenge and progression of infection, the SPIPm2 was secreted readily from the semigranular and granular hemocytes. The more they secreted the SPIPm2, the less they were distinguishable from the hyaline cells. The WSSV-infected cells were either semigranular or granular hemocytes or both and depleted of SPIPm2. The rSPIPm2 was able to temporarily and dose-dependently neutralize the WSSV and protect the hemocytes from viral infection judging from the substantially less expression of WSSV late gene VP28. The antiviral activity was very likely due to the binding of SPIPm2 to the components of viral particle and hemocyte cell membrane.
一种 Kazal 型丝氨酸蛋白酶 SPIPm2 在对虾血淋巴中大量表达，并被证明参与了对虾对白斑综合征病毒（WSSV）的先天免疫反应。SPIPm2 在半颗粒和颗粒状细胞质的颗粒中表达和储存，但不在透明细胞质中表达和储存。在 WSSV 感染和感染进展过程中，SPIPm2 容易从半颗粒和颗粒状血细胞中分泌出来。它们分泌的 SPIPm2 越多，它们与透明细胞的区别就越小。受 WSSV 感染的细胞要么是半颗粒状或颗粒状血细胞，要么两者兼而有之，并且耗尽了 SPIPm2。rSPIPm2 能够暂时和剂量依赖性地中和 WSSV，并从病毒感染中保护血细胞，从实质上减少了 WSSV 晚期基因 VP28 的表达。抗病毒活性很可能是由于 SPIPm2 与病毒粒子和血细胞细胞膜的成分结合。
