通过在膜上进行快速免疫迁移诊断犬细小病毒

Diagnosis of canine parvovirus by rapid immunomigration on a membrane.

作者信息

Lacheretz A, Laperrousaz C, Kodjo A, Brajon N, Crevat D, Guillossou S

机构信息

Infectious Pathology Unit, Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat, BP 83, 69280 Marcy l'Etoile, France.

出版信息

Vet Rec. 2003 Jan 11;152(2):48-50. doi: 10.1136/vr.152.2.48.

DOI:10.1136/vr.152.2.48

PMID:12553581

Abstract

Rapid immunomigration on a membrane was applied to the diagnosis of canine parvovirus (CPV) in 128 samples of faeces containing four strains of parvovirus (two CPV-2a strains, including one vaccine strain, and two CPV-2b strains). The results were compared with the results of haemagglutination and ELISA sandwich techniques. The new test was quick and easy to use, and made it possible to identify both the CPV-2a and CPV-2b strains. Its detection thresholds per gram of faeces corresponded to specific haemagglutination titres of between 320 and 640 and a virus titre of between 10(4) and 10(5) CCID50 (dose required to infect 50 per cent of cell cultures).

摘要

采用膜上快速免疫迁移法对128份含有4株细小病毒（2株CPV - 2a毒株，其中包括1株疫苗株，以及2株CPV - 2b毒株）的粪便样本进行犬细小病毒（CPV）诊断。将结果与血凝和ELISA夹心技术的结果进行比较。这项新检测方法快速且易于使用，能够鉴别CPV - 2a和CPV - 2b毒株。其每克粪便的检测阈值相当于320至640的特异性血凝滴度以及10(4)至10(5) CCID50（感染50%细胞培养物所需剂量）的病毒滴度。