Drane D P, Hamilton R C, Cox J C
Research and Development Division, CSL Limited, Parkville, Vic., Australia.
Vet Microbiol. 1994 Aug 1;41(3):293-302. doi: 10.1016/0378-1135(94)90109-0.
The CPV ELISA detection kit (CSL Ltd., Melbourne Australia) was developed as a rapid field test for the detection of canine parvovirus (CPV) antigen in canine faecal samples. The kit utilises a novel concept in enzyme immunoassay (ELISA) where the conjugate is lyophilised along with the solid-phase capture antibody in the test well. The CPV ELISA was compared to the haemagglutination assay (HA) test using electron microscopy (EM) and/or virus isolation (VI) to confirm infection. The CPV ELISA had a sensitivity of 87% and a specificity of 100% compared to 87% and 63% respectively for the HA. The poor specificity of the HA results in a low positive predictive value of 51% compared to 100% for the CPV ELISA. The CPV ELISA requires no specialised equipment, is simple to perform and provides a visible result in less than 15 minutes.
犬细小病毒（CPV）酶联免疫吸附测定（ELISA）检测试剂盒（澳大利亚墨尔本CSL有限公司）是作为一种用于检测犬粪便样本中犬细小病毒（CPV）抗原的快速现场检测方法而开发的。该试剂盒在酶免疫测定（ELISA）中采用了一种新颖的概念，即结合物与固相捕获抗体一起在测试孔中冻干。将CPV ELISA与血凝试验（HA）进行比较，并使用电子显微镜（EM）和/或病毒分离（VI）来确认感染。与HA分别为87%和63%的灵敏度和特异性相比，CPV ELISA的灵敏度为87%，特异性为100%。HA的低特异性导致其阳性预测值较低，为51%，而CPV ELISA的阳性预测值为100%。CPV ELISA不需要专门设备，操作简单，且能在不到15分钟内给出可见结果。
