Shakoor K A, Saleh A, Khanzada M S
Department of Pathology, BMSI, Jinnah Postgraduate Medical Centre, Karachi.
J Pak Med Assoc. 2002 Oct;52(10):442-7.
To identify Hodgkin's and Reed Sternberg like cells by a single Immunomarker (Ki-1; CD-30) and to highlight diagnostic specificity of these cells in Hodgkin's disease, as these cells are frequently encountered in other lymphoproliferative disorders on H&E sections.
Seventy-nine histologically diagnosed cases of lymph node biopsies (59 cases of Hodgkin's disease, 15 cases of Non Hodgkin's lymphoma and 5 cases of non neoplastic lymphoid tissue) were subjected to Ki-1 (CD-30) immunostaining as all cases revealed Hodgkin's and/or Reed Sternberg like cells on H&E stained sections.
Out of 59 cases of Hodgkin's disease, 49 (83%) showed Ki-1 (CD-30) immunoreactivity for Reed Sternberg and Hodgkin's cells. None of the 15 cases of non-Hodgkin's lymphoma (2 small lymphocyte type, 11 diffuse large cells type, 2 lymphoblastic type) showed positive Ki-1 (CD-30) immunostaining. Only one case of the remaining 5 cases of non-neoplastic lymphoid lesion (2 toxoplasmic lymphadenitis, one histiocytic necrotizing lymphadenitis, one reactive hyperplasia and one chronic non specific lymphadenitis) showed positive Ki-1 immunostaining.
Ki-1 (CD-30) is a reliable marker for Hodgkin's Reed Sternberg like cells in histologically proven cases of Hodgkin's disease but not for similar cells encountered in other lymhoproliferative lesions.
通过单一免疫标志物（Ki-1；CD-30）识别霍奇金和里德-斯腾伯格样细胞，并强调这些细胞在霍奇金病中的诊断特异性，因为在苏木精-伊红（H&E）切片上，这些细胞在其他淋巴增殖性疾病中也经常出现。
79例经组织学诊断的淋巴结活检病例（59例霍奇金病、15例非霍奇金淋巴瘤和5例非肿瘤性淋巴组织）进行了Ki-1（CD-30）免疫染色，因为所有病例在H&E染色切片上均显示有霍奇金和/或里德-斯腾伯格样细胞。
在59例霍奇金病病例中，49例（83%）的里德-斯腾伯格细胞和霍奇金细胞显示Ki-1（CD-30）免疫反应性。15例非霍奇金淋巴瘤（2例小淋巴细胞型、11例弥漫大细胞型、2例淋巴母细胞型）均未显示Ki-1（CD-30）免疫染色阳性。其余5例非肿瘤性淋巴病变（2例弓形虫淋巴结炎、1例组织细胞坏死性淋巴结炎、1例反应性增生和1例慢性非特异性淋巴结炎）中只有1例显示Ki-1免疫染色阳性。
在组织学确诊的霍奇金病病例中，Ki-1（CD-30）是霍奇金里德-斯腾伯格样细胞的可靠标志物，但对于其他淋巴增殖性病变中出现的类似细胞则不可靠。
