Schlegelová J, Dendis M, Benedík J, Babák V, Rysánek D
Department of Dairy Science, Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic.
Vet Microbiol. 2003 Apr 29;92(4):327-34. doi: 10.1016/s0378-1135(02)00409-1.
Staphylococcus aureus is a frequent cause of animal and human infections. The aim of the present study was to test diversity of the populations of S. aureus colonising cattle and humans sharing an infected environment. Eighty-six S. aureus isolates obtained from dairy cows, from people coming into contact with dairy cows on the farm and the other farm personnel were characterized by restriction fragment length polymorphism of the coagulase gene. Molecular analyses identified ten polymorphism types with prevalent presentation of type II in isolates from cow's milk and type IV in isolates from people coming into contact with dairy cows on the farm (the cattlemen) and the other farm personnel. Seven further genotypes were identified among the isolates from the cattlemen. The results indicate that the strains dominating in human population did not equate to the causative agents of bovine mastitis.
金黄色葡萄球菌是动物和人类感染的常见病因。本研究的目的是检测在共享感染环境的牛和人类中定植的金黄色葡萄球菌种群的多样性。从奶牛、农场中接触奶牛的人员以及其他农场工作人员中获得的86株金黄色葡萄球菌分离株，通过凝固酶基因的限制性片段长度多态性进行了特征分析。分子分析确定了10种多态性类型，其中牛奶分离株中II型占主导，农场中接触奶牛的人员（牧民）和其他农场工作人员的分离株中IV型占主导。在牧民的分离株中还鉴定出另外7种基因型。结果表明，在人类群体中占主导的菌株与牛乳腺炎的病原体并不相同。
