Su C, Herbelin C, Frieze N, Skardova O, Sordillo L M
Department of Veterinary Science, Center for Mastitis Research, The Pennsylvania State University, University Park 16802-3500, USA.
Epidemiol Infect. 1999 Apr;122(2):329-36. doi: 10.1017/s0950268899002228.
The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P < 0.01) differences in the average percent neutrophil killing of the predominant (16.7%) and rare (39.7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes.
本研究的目的是调查从患有乳腺炎的牛乳中分离出的金黄色葡萄球菌凝固酶基因多态性，并确定主要和罕见凝固酶基因型对牛血中性粒细胞杀菌活性的抗性。总共从四个国家收集了453株分离株：捷克共和国、法国、韩国和美国。通过凝固酶基因的限制性片段长度多态性（RFLP）将分离株分为40种类型。对来自主要和罕见基因型的23株菌株抵抗中性粒细胞杀菌活性的能力进行了评估。当用抗血清调理细菌时，主要基因型（16.7%）和罕见基因型（39.7%）的中性粒细胞平均杀伤百分比存在显著（P < 0.01）差异。结果表明，凝固酶基因型谱在不同地理位置有所不同，且每个地点只有少数基因型占主导。此外，主要基因型比罕见基因型对中性粒细胞杀菌活性更具抗性。
