小鼠获得性免疫缺陷综合征及长期乙醇摄入时血小板CD62p表达与微粒情况
Platelet CD62p expression and microparticle in murine acquired immune deficiency syndrome and chronic ethanol consumption.
作者信息
Chen Yinhong, Davis-Gorman Grace, Watson Ronald R, McDonagh Paul F
机构信息
Divison of Health Promotion Science, College of Public Health, College of Medicine and The Sarver Heart Center, University of Arizona, Tucson, AZ 85724, USA.
出版信息
Alcohol Alcohol. 2003 Jan-Feb;38(1):25-30. doi: 10.1093/alcalc/agg013.
AIMS
Abnormal platelet counts have been noticed in acquired immune deficiency syndrome (AIDS) patients. However, the actual state of platelets in AIDS is unclear. We hypothesize that platelets are activated and platelet-derived microparticles increase in murine AIDS.
METHODS
To elucidate the ethanol effects on platelets in murine AIDS, we studied four groups: control, murine AIDS, ethanol, and ethanol plus murine AIDS. Platelet CD62p as a platelet activation marker and CD61(+) microparticles as platelet microparticles (PMPs) were measured by flow cytometry.
RESULTS
Platelets were significantly activated in mice with murine AIDS and chronic ethanol consumption. Increased platelet CD62p expression and increased PMPs were most pronounced in advanced stages of murine AIDS. Chronic ethanol consumption persistently enhanced platelet activation and PMP formation.
CONCLUSIONS
Elevated platelet CD62p and PMPs may represent a pro-thrombotic status that have important pathological consequences.
目的
在获得性免疫缺陷综合征（艾滋病）患者中已发现血小板计数异常。然而，艾滋病中血小板的实际状态尚不清楚。我们推测在小鼠艾滋病模型中血小板被激活且血小板衍生微粒增加。
方法
为阐明乙醇对小鼠艾滋病模型中血小板的影响，我们研究了四组：对照组、小鼠艾滋病模型组、乙醇组以及乙醇加小鼠艾滋病模型组。通过流式细胞术检测作为血小板活化标志物的血小板CD62p以及作为血小板微粒（PMPs）的CD61(+)微粒。
结果
在患有小鼠艾滋病模型且长期摄入乙醇的小鼠中，血小板被显著激活。血小板CD62p表达增加以及PMPs增加在小鼠艾滋病模型晚期最为明显。长期摄入乙醇持续增强血小板活化和PMPs形成。
结论
血小板CD62p和PMPs升高可能代表一种具有重要病理后果的促血栓状态。
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