Ono Takeshi, Tadakuma Takushi, Rodriguez Ana
New York University School of Medicine, Department of Medical Parasitology, New York, NY 10010, USA.
Exp Parasitol. 2007 Mar;115(3):310-3. doi: 10.1016/j.exppara.2006.09.008. Epub 2006 Oct 17.
Plasmodium yoelii is a rodent parasite commonly used as a model to study malaria infection. It is the preferred model parasite for liver-stage immunological studies and is also widely used to study hepatocyte, erythrocyte and mosquito infection. We have generated a P. yoelii yoelii 17XNL line that is stably transfected with the green fluorescent protein (gfp) gene. This parasite line constitutively expresses high levels of GFP during the complete parasite life cycle including liver, blood and mosquito stages. These fluorescent parasites can be used in combination with fluorescence activated cell sorting or live microscopy for a wide range of experimental applications.
约氏疟原虫是一种啮齿动物寄生虫，通常用作研究疟疾感染的模型。它是肝期免疫学研究的首选模型寄生虫，也广泛用于研究肝细胞、红细胞和蚊虫感染。我们构建了一个稳定转染绿色荧光蛋白（gfp）基因的约氏疟原虫17XNL品系。该寄生虫品系在包括肝、血液和蚊虫阶段的整个寄生虫生命周期中持续高水平表达GFP。这些荧光寄生虫可与荧光激活细胞分选或活细胞显微镜结合使用，用于广泛的实验应用。
