[Cloning and sequencing of cDNA from G protein gene of subgroup B respiratory syncytial virus strain isolated in China].

The nucleotide sequence of the G protein gene of respiratory syncytial virus (RSV) CC169 strain isolated from China that has been identified as subgroup B with monoclonal antibodies, was determined from cDNA that had been amplified by RT-PCR and cloned into pTZ18R plasmid vector. The homology of nucleotide was 94% as compared with G protein cDNA of a RSV prototype strain (CH18537). Deduced amino acid identity of G protein was 89.4%. The amino acid changes were only in the extracellular part of the protein where there were two extensive divergent domains with a highly conserved region in between; whereas the cytoplasmic and transmembrane domains were conserved. This study demonstrates the sequence diversity of the G protein of subgroup B RSV between a Chinese isolate and the prototype strain CH18537.