Wu Mianbin, Xia Liming
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
Wei Sheng Wu Xue Bao. 2002 Jun;42(3):364-9.
Both laccase production by the white-rot fungus Coriolus versicolor and decolorization of dyestuff and dying waste water with crude solution of laccase were studied in this work. Laccase production meets the definition of secondary metabolism. For laccase production the optimum initial pH is 4.5. Addition of veratryl alcohol or elevated trace metals could both enhance the laccase activity, while Tween80 showed some inhibition. The immobilized mycelia of C. versicolor in polyurethane foam had less laccase production ability than mycelial pellets. A repeated batch cultivation process was found to be a very economical way for laccase harvest. The same pellets could be used for at least 14 times and average laccase activity of each batch could maintain 6.72 IU/mL. This method reduces the enzyme production course, medium consumption and the possibility of contamination, showing high efficient and great economic benefit. Good results were also obtained in decolorization experiments with the crude solution of laccase. With 3.3 IU/mL initial laccase activity, color removal of Acid Orange reached 98.5% after 24 h reaction. Also with 2.6 IU/mL initial laccase activity, color removal of dying waste water reached 93% after 24 h reaction.
本研究对白腐真菌云芝产漆酶以及漆酶粗溶液对染料和印染废水的脱色进行了研究。漆酶的产生符合次级代谢的定义。产漆酶的最适初始pH为4.5。添加藜芦醇或提高微量金属含量均可提高漆酶活性，而吐温80则表现出一定的抑制作用。固定在聚氨酯泡沫中的云芝菌丝体产漆酶能力低于菌丝球。发现重复分批培养过程是收获漆酶非常经济的方法。同样的菌丝球可使用至少14次，每批的平均漆酶活性可维持在6.72 IU/mL。该方法缩短了酶的生产过程，减少了培养基消耗和污染可能性，具有高效性和显著的经济效益。漆酶粗溶液的脱色实验也取得了良好结果。初始漆酶活性为3.3 IU/mL时，反应24 h后酸性橙的脱色率达到98.5%。初始漆酶活性为2.6 IU/mL时，印染废水反应24 h后的脱色率达到93%。
