Lorenzo Miriam, Moldes Diego, Sanromán M A Angeles
Department of Chemical Engineering, University of Vigo, Campus Universitario As Lagoas-Marcosende, E-36310 Vigo, Spain.
Chemosphere. 2006 May;63(6):912-7. doi: 10.1016/j.chemosphere.2005.09.046. Epub 2005 Nov 15.
The white-rot fungus Trametes versicolor growing in submerged culture on a basal medium, with barley bran as a carbon source, produced two laccase isoenzymes LacI and LacII. The addition of metal ions to the culture medium was performed to improve the total laccase activity and to determine the effect on the production of laccase isoenzymes. From all the tested metals, only Cu2+ increased laccase activity (up to 12-fold with respect to control cultures) and T. versicolor in presence of all metals produced the two isoenzymes in different proportion with ratios of activity (LacI/LacII) varying between 0.11 and 0.51. This factor played an important role in the decolourisation of the textile dye Indigo Carmine.
在以大麦麸作为碳源的基础培养基中进行液体培养的白腐真菌云芝，产生了两种漆酶同工酶LacI和LacII。向培养基中添加金属离子，以提高漆酶的总活性，并确定其对漆酶同工酶产生的影响。在所有测试的金属中，只有Cu2+提高了漆酶活性（相对于对照培养物提高了12倍），并且在所有金属存在的情况下，云芝产生两种同工酶的比例不同，活性比（LacI/LacII）在0.11至0.51之间变化。该因素在纺织染料靛蓝胭脂红的脱色中起重要作用。
