Ying Peiqing, Jin Gang, Tao Zulai
National Microgravity Lab, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2002 Sep;19(3):370-3.
Cell adhesion to material surface plays an important role in regulating cell function such as proliferation and differentiation. Surface patterning provides a useful method to control cell spatial distribution and adhesion to substance. Here microcontact printing and microfluidic channels were introduced to pattern MC3T3 E1 osteoblasts on silicon substance. Dichlordimethylsilane (DMS) was used in microcontact printing to generate the alternating domains of DMS and non-DMS, and cells preferentially adhered to the non-DMS and hydrophilic region. On the patterned surfaces generated from collagen and albumin solutions with microfluidic channels, cells preferentially localized in the collagen-coated region. The results also showed that micropatterning could be a useful method to study the effect of surface chemistry on cell adhesion and other functions.
细胞对材料表面的黏附在调节细胞功能（如增殖和分化）中起着重要作用。表面图案化提供了一种控制细胞空间分布和对物质黏附的有用方法。在此，微接触印刷和微流控通道被用于在硅物质上对MC3T3 E1成骨细胞进行图案化处理。微接触印刷中使用二氯二甲基硅烷（DMS）来产生DMS和非DMS的交替区域，细胞优先黏附于非DMS和亲水区域。在由胶原蛋白和白蛋白溶液通过微流控通道产生的图案化表面上，细胞优先定位在胶原蛋白包被的区域。结果还表明，微图案化可能是一种研究表面化学对细胞黏附和其他功能影响的有用方法。
