Kawashita Masakazu, Hayashi Jumpei, Kudo Tada-aki, Kanetaka Hiroyasu, Li Zhixia, Miyazaki Toshiki, Hashimoto Masami
Graduate School of Biomedical Engineering, Tohoku University, 6-6-11-901-4 Aramaki-Aoba, Aoba-ku, Sendai, 980-8579, Japan.
J Biomed Mater Res A. 2014 Jun;102(6):1880-6. doi: 10.1002/jbm.a.34861. Epub 2013 Jul 24.
Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.
植入后的初始细胞反应对于诱导骨传导性很重要。我们使用小鼠MC3T3-E1成骨细胞和小鼠RAW264.7巨噬细胞，研究了细胞对天然和牛血清白蛋白（BSA）吸附的羟基磷灰石（HA）或α型氧化铝（α-Al2O3）圆盘的粘附、铺展和增殖情况。吸附的BSA抑制了MC3T3-E1细胞的粘附和铺展，但不影响MC3T3-E1细胞在HA和α-Al2O3基质上的增殖。因此，在细胞结合被足以抑制MC3T3-E1细胞粘附的BSA吸附阻碍之前，MC3T3-E1细胞能快速粘附到原始HA上。吸附的BSA抑制RAW264.7细胞对α-Al2O3的粘附，但不抑制对HA的粘附。BSA吸附不影响RAW264.7细胞在HA和α-Al2O3基质上的铺展和增殖。因此，吸附在HA上的BSA刺激的细胞反应与α-Al2O3不同。此外，成骨细胞和单核细胞-巨噬细胞谱系细胞的快速粘附在HA的骨传导性中起作用。
