Suto Robert K, Edayathumangalam Rajeswari S, White Cindy L, Melander Christian, Gottesfeld Joel M, Dervan Peter B, Luger Karolin
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
J Mol Biol. 2003 Feb 14;326(2):371-80. doi: 10.1016/s0022-2836(02)01407-9.
We determined the crystal structures of three nucleosome core particles in complex with site-specific DNA-binding ligands, the pyrrole-imidazole polyamides. While the structure of the histone octamer and its interaction with the DNA remain unaffected by ligand binding, nucleosomal DNA undergoes significant structural changes at the ligand-binding sites and in adjacent regions to accommodate the ligands. Our findings suggest that twist diffusion occurs over long distances through tightly bound nucleosomal DNA. This may be relevant to the mechanism of ATP-dependent and spontaneous nucleosome translocation, and to the effect of bound factors on nucleosome dynamics.
我们确定了三种核小体核心颗粒与位点特异性DNA结合配体（即吡咯-咪唑聚酰胺）形成复合物的晶体结构。虽然组蛋白八聚体的结构及其与DNA的相互作用不受配体结合的影响，但核小体DNA在配体结合位点及相邻区域会发生显著的结构变化以容纳配体。我们的研究结果表明，扭曲扩散通过紧密结合的核小体DNA在长距离上发生。这可能与ATP依赖的和自发的核小体易位机制以及结合因子对核小体动力学的影响有关。
