Höller Ulrich, Brodhag Christine, Knöbel Andreas, Hofmann Peter, Spitzer Volker
Roche Vitamins Ltd, Human Nutrition and Health, Analytics (VFHA), CH-4070 Basel, Switzerland.
J Pharm Biomed Anal. 2003 Feb 5;31(1):151-8. doi: 10.1016/s0731-7085(02)00574-5.
The determination of folic acid, nicotinamide, nicotinic acid, riboflavin, riboflavin-5'-phosphate, pyridoxine and thiamine in tablets has been successfully automated using a bench-top robotic system (Zymark Tablet Processing Workstation II) coupled to reversed-phase (RP-18) HPLC with UV-detection. The new automated methods have been validated and were found to be applicable in routine analysis for all common types of multivitamin tablets. The precision is exemplified by relative standard deviations of 5.4% for folic acid, 2.1% for nicotinamide, 1.6% for pyridoxine, 0.7% for riboflavin and 1.1% for thiamine using film-coated tablets as sample. Mean recoveries obtained during spiking experiments were in the range of 95.2-103.9%.
使用台式机器人系统（Zymark片剂处理工作站II）与配有紫外检测的反相（RP - 18）高效液相色谱联用，已成功实现了片剂中叶酸、烟酰胺、烟酸、核黄素、核黄素-5'-磷酸、吡哆醇和硫胺素的自动化测定。新的自动化方法已经过验证，发现适用于所有常见类型多种维生素片剂的常规分析。以薄膜包衣片为样品时，叶酸的相对标准偏差为5.4%，烟酰胺为2.1%，吡哆醇为1.6%，核黄素为0.7%，硫胺素为1.1%，体现了该方法的精密度。加标实验中获得的平均回收率在95.2 - 103.9%范围内。
