van der Horst A, Martens H J, de Goede P N
Department of Pharmacy, Free University, Amsterdam, The Netherlands.
Pharm Weekbl Sci. 1989 Oct 20;11(5):169-74. doi: 10.1007/BF01959466.
A stability-indicating high pressure liquid chromatographic method was developed to determine the stability of water-soluble vitamins in total parenteral nutrition mixtures. Folic acid and thiamine were determined by direct injection and precolumn enrichment, followed by UV detection. Nicotinamide and pyridoxine were determined simultaneously without sample pretreatment by UV detection of nicotinamide and fluorescence detection of pyridoxine. Riboflavin 5'-phosphate was also determined without elaborate sample handling and by fluorescence detection. Ascorbic acid was determined as the sum of ascorbic acid and dehydroascorbic acid. After derivatization to a quinoxaline the latter substance was determined by direct injection and fluorescence detection.
建立了一种稳定性指示高压液相色谱法，用于测定全胃肠外营养混合物中水溶性维生素的稳定性。叶酸和硫胺素通过直接进样和柱前富集，随后进行紫外检测来测定。烟酰胺和吡哆醇通过对烟酰胺进行紫外检测以及对吡哆醇进行荧光检测，无需样品预处理即可同时测定。核黄素5'-磷酸也无需复杂的样品处理，通过荧光检测来测定。抗坏血酸以抗坏血酸和脱氢抗坏血酸的总和来测定。在衍生化为喹喔啉后，通过直接进样和荧光检测来测定后一种物质。
