Shemarova I V, Selivanova G V, Vlasova T D
Institute of Evolutionary Physiology and Biochemistry RAS, St. Petersburg.
Tsitologiia. 2002;44(11):1097-103.
The influence of the epidermal growth factor (EGE) (10(-8) M), insulin (10(-6) M) and EGF (10(-8) M) in combination with insulin (10(-6) M) on proliferation and DNA synthesis in the nuclei of ciliates Tetrahymena pyriformis GL was studied. Insulin and EGF, known to stimulate growth of many types of mammalian cells revealed a mitogen influence on the unicellular eukaryotes. This effect involves stimulation of DNA synthesis, rising synchronization of cell division (upon the influence of EGF), and increase in cell number during the exponential growth. The mitogen effect may be evoked by cell progression in G1-phase under the action of growth factors and, consequently by earlier entry of cells into S-phase of the first cell cycle. Insulin repressed division of cells that entered into the generative cycle. These cells were delayed in late S-phase and G2-phase of the cycle. Part of these cells perished, while other cells could successively overcome the cell block to start their division by the 4th hours of cultivation. A collateral cytotoxic effect of insulin was found, being most prominent in early periods of Tetrahymena cultivation.
研究了表皮生长因子（EGE）（10^(-8) M）、胰岛素（10^(-6) M）以及表皮生长因子（10^(-8) M）与胰岛素（10^(-6) M）联合使用对梨形四膜虫GL细胞核增殖和DNA合成的影响。已知胰岛素和表皮生长因子可刺激多种哺乳动物细胞生长，它们对单细胞真核生物也显示出有丝分裂原的影响。这种效应包括刺激DNA合成、提高细胞分裂同步性（在表皮生长因子影响下）以及在指数生长期增加细胞数量。有丝分裂原效应可能是由生长因子作用下细胞在G1期的进程引发的，从而导致细胞更早进入第一个细胞周期的S期。胰岛素抑制进入生殖周期的细胞分裂。这些细胞在周期的S期后期和G2期被延迟。部分细胞死亡，而其他细胞能够相继克服细胞阻滞，在培养4小时后开始分裂。发现胰岛素有附带的细胞毒性作用，在四膜虫培养早期最为显著。
