Csaba G, Kovács P, Falus A
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Cytokine. 1995 Nov;7(8):771-4. doi: 10.1006/cyto.1995.0092.
Interleukin (IL)-3 and IL-6 significantly increase the growth rate of the unicellular organism, Tetrahymena. The effect elicited by IL-3 is long lasting as it was also detectable after 20 generations. Effect of IL-6 was detectable as long as the substance was present in the cell culture. Pretreatment with IL-3 did not enhance the proliferative response to subsequent IL-3 treatment, but the second exposure to IL-3 considerably depressed the active proliferation of Tetrahymena cells. However, a positive 'priming effect' elicited by IL-6 resulted in an increased growth rate following repeated IL-6 stimulation. Insulin binding to the plasma membrane of Tetrahymena was increased by IL-6 but not by IL-3 after 24 hours, and this enhancement appeared even after one hour incubation. If the cells were pretreated with insulin, IL-6 did not influence insulin binding, while an inhibition by IL-3 was observed. These results direct attention to the similarities of actions induced by IL-3 and IL-6 at different levels of phylogeny probably due to the presence of cytokine receptor-like structures on this unicellular organism.
白细胞介素（IL）-3和IL-6可显著提高单细胞生物四膜虫的生长速率。IL-3所引发的效应具有持久性，因为在20代之后仍可检测到。只要细胞培养物中存在IL-6，其效应就能被检测到。用IL-3预处理不会增强对后续IL-3处理的增殖反应，但再次接触IL-3会显著抑制四膜虫细胞的活跃增殖。然而，IL-6引发的正向“启动效应”会导致在反复IL-6刺激后生长速率增加。24小时后，IL-6可增加胰岛素与四膜虫质膜的结合，但IL-3则无此作用，且这种增强在孵育1小时后就已出现。如果用胰岛素对细胞进行预处理，IL-6不会影响胰岛素结合，而IL-3则会产生抑制作用。这些结果使人们将注意力转向IL-3和IL-6在不同进化水平上所诱导的作用的相似性，这可能是由于这种单细胞生物上存在细胞因子受体样结构所致。
