Khalifa A M
Department of Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
J Egypt Soc Parasitol. 1999;29(1):157-65.
One hundred and fifty stool samples were collected from diarrheic patients of different ages, and examined for Blastocystis hominis by direct smears and concentrated by Sheather's sugar flotation. Staining was done by: Giemsa, two modifications of trichrome stain, modified Ziehl-Neelsen, safranin-methylene blue and two-auramine stains. Out of the 150 cases nine were positive for blastocystosis. The best stains were safranin-methylene blue and modified Ziehl-Neelsen stains. They had the advantage of staining cysts and amoeboid forms besides being rapid and easy to perform. The modified trichrome stains identified 8 ie, less specific and were time consuming. The auramine dyes stained the cyst, both the wall and internal body fluoresced brightly. Giemsa stain was not an efficient stain. Scanning and transmission electron microscopy (SEM, TEM) were performed to study the fine ultrastructure.
从不同年龄段的腹泻患者中收集了150份粪便样本，通过直接涂片法检测人芽囊原虫，并采用谢弗氏糖浮选法进行浓缩。染色方法如下：吉姆萨染色、两种改良三色染色、改良齐-尼氏染色、番红-亚甲蓝染色和两种金胺染色。150例病例中有9例人芽囊原虫病呈阳性。最佳染色方法是番红-亚甲蓝染色和改良齐-尼氏染色。它们除了快速且易于操作外，还具有能对囊肿和阿米巴样形态进行染色的优点。改良三色染色法识别出8例，即特异性较低且耗时。金胺染料能使囊肿染色，囊肿壁和内部主体均发出明亮荧光。吉姆萨染色不是一种有效的染色方法。进行了扫描电子显微镜和透射电子显微镜（SEM、TEM）检查以研究精细超微结构。
