短期体外培养在粪便标本中检测人芽囊原虫及分子研究中的应用价值。
The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens.
作者信息
Termmathurapoj Sumeth, Leelayoova Saovanee, Aimpun Pote, Thathaisong Umaporn, Nimmanon Thirayost, Taamasri Paanjit, Mungthin Mathirut
机构信息
Department of Pathology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand.
出版信息
Parasitol Res. 2004 Aug;93(6):445-7. doi: 10.1007/s00436-004-1157-x. Epub 2004 Jul 9.
When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.
当体外培养被用作粪便标本中检测人芽囊原虫的“金标准”时，简单涂片和三色染色的敏感性分别为16.7%和40.2%，特异性分别为94%和80.4%。体外培养还增强了粪便标本中人芽囊原虫检测的PCR扩增。我们的数据表明体外培养在粪便标本中人芽囊原虫检测和分子研究中的有用性。
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