Rédly Gyula Alan, Poole Keith
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
J Bacteriol. 2003 Feb;185(4):1261-5. doi: 10.1128/JB.185.4.1261-1265.2003.
A search of the pvd pyoverdine biosynthesis locus of Pseudomonas aeruginosa identified an open reading frame, PA2387, whose product exhibited a sequence similar to those of a number of so-called extracytoplasmic- function sigma factors responsible for siderophore-dependent expression of iron-siderophore receptors in Escherichia coli and Pseudomonas putida. Deletion of this gene, dubbed fpvI, compromised pyoverdine-dependent FpvA ferric pyoverdine receptor production and fpvA gene expression, while the cloned gene stimulated fpvA expression. A Fur-binding site was identified immediately upstream of fpvI, consistent with the observed iron-regulated expression of fpvI and fpvA.
对铜绿假单胞菌的绿脓菌素生物合成基因座进行搜索时，发现了一个开放阅读框PA2387，其产物的序列与一些所谓的胞外功能西格玛因子相似，这些因子负责大肠杆菌和恶臭假单胞菌中铁载体依赖性铁-铁载体受体的表达。缺失这个被命名为fpvI的基因，会损害绿脓菌素依赖性FpvA铁绿脓菌素受体的产生以及fpvA基因的表达，而克隆的该基因则会刺激fpvA的表达。在fpvI的紧邻上游发现了一个Fur结合位点，这与观察到的fpvI和fpvA的铁调节表达一致。
