2-氨基嘌呤通过可逆性失活大肠杆菌错配修复系统实现种间重组。
2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.
作者信息
Matic Ivan, Babic Ana, Radman Miroslav
机构信息
INSERM U571, Faculté de Médecine, Necker-Enfants Malades, Université René Descartes, 75730 Paris Cedex 15, France.
出版信息
J Bacteriol. 2003 Feb;185(4):1459-61. doi: 10.1128/JB.185.4.1459-1461.2003.
Abstract
2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.
摘要
用2-氨基嘌呤处理大肠杆菌可诱导出DNA错配修复缺陷的可逆表型。这种短暂的表型导致与肠炎沙门氏菌鼠伤寒血清型Hfr供体的种间接合重组频率增加300倍。该方法可通过允许分化的基因和基因组之间进行重组来用于生物多样性的产生。
相似文献
1
2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.2-氨基嘌呤通过可逆性失活大肠杆菌错配修复系统实现种间重组。
J Bacteriol. 2003 Feb;185(4):1459-61. doi: 10.1128/JB.185.4.1459-1461.2003.
2
Mechanism and control of interspecies recombination in Escherichia coli. I. Mismatch repair, methylation, recombination and replication functions.大肠杆菌种间重组的机制与调控。I.错配修复、甲基化、重组与复制功能。
Genetics. 1998 Oct;150(2):533-42. doi: 10.1093/genetics/150.2.533.
3
Interspecies recombination and mismatch repair. Generation of mosaic genes and genomes.种间重组与错配修复。镶嵌基因和基因组的产生。
Methods Mol Biol. 2000;152:149-57. doi: 10.1385/1-59259-068-3:149.
4
Structure of recombinants from conjugational crosses between Escherichia coli donor and mismatch-repair deficient Salmonella typhimurium recipients.大肠杆菌供体与错配修复缺陷型鼠伤寒沙门氏菌受体之间通过接合杂交产生的重组体结构。
Genetics. 1994 Jan;136(1):17-26. doi: 10.1093/genetics/136.1.17.
5
Multicopy single-stranded DNA of Escherichia coli enhances mutation and recombination frequencies by titrating MutS protein.大肠杆菌的多拷贝单链DNA通过滴定MutS蛋白提高突变和重组频率。
Mol Microbiol. 1996 Feb;19(3):505-9. doi: 10.1046/j.1365-2958.1996.392921.x.
6
In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination.MutS、MutL和MutH突变体的体外和体内研究：错配修复与DNA重组的相关性
DNA Repair (Amst). 2003 Apr 2;2(4):387-405. doi: 10.1016/s1568-7864(02)00245-8.
7
Evolutionary implications of the frequent horizontal transfer of mismatch repair genes.错配修复基因频繁水平转移的进化意义
Cell. 2000 Nov 22;103(5):711-21. doi: 10.1016/s0092-8674(00)00175-6.
8
The coordinated functions of the E. coli MutS and MutL proteins in mismatch repair.大肠杆菌错配修复中MutS和MutL蛋白的协同功能。
Mol Cell. 2003 Jul;12(1):233-46. doi: 10.1016/s1097-2765(03)00219-3.
9
The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants.大肠杆菌和鼠伤寒沙门氏菌之间重组的障碍在错配修复突变体中被打破。
Nature. 1989 Nov 23;342(6248):396-401. doi: 10.1038/342396a0.
10
Wot the 'L-Does MutL do?MutL 是做什么的？
Mutat Res. 2010 Dec;705(3):228-38. doi: 10.1016/j.mrrev.2010.07.002. Epub 2010 Aug 3.
引用本文的文献
1
Genome-wide mapping of spontaneous DNA replication error-hotspots using mismatch repair proteins in rapidly proliferating Escherichia coli.利用错配修复蛋白在快速增殖的大肠杆菌中对自发DNA复制错误热点进行全基因组定位。
Nucleic Acids Res. 2025 Jan 11;53(2). doi: 10.1093/nar/gkae1196.
2
Real-time monitoring of replication errors' fate reveals the origin and dynamics of spontaneous mutations.实时监测复制错误的命运揭示了自发突变的起源和动态。
Nat Commun. 2024 Mar 27;15(1):2702. doi: 10.1038/s41467-024-46950-0.
3
Recombineering and MAGE.重组工程与多重自动基因组工程技术
Nat Rev Methods Primers. 2021;1. doi: 10.1038/s43586-020-00006-x. Epub 2021 Jan 14.
4
Mismatch Repair: From Preserving Genome Stability to Enabling Mutation Studies in Real-Time Single Cells.错配修复：从保护基因组稳定性到实时单细胞突变研究。
Cells. 2021 Jun 18;10(6):1535. doi: 10.3390/cells10061535.
5
Mutagen Synergy: Hypermutability Generated by Specific Pairs of Base Analogs.诱变剂协同作用：特定碱基类似物对产生的高突变性
J Bacteriol. 2016 Sep 22;198(20):2776-83. doi: 10.1128/JB.00391-16. Print 2016 Oct 15.
6
Stoichiometry of MutS and MutL at unrepaired mismatches in vivo suggests a mechanism of repair.体内未修复错配物处 MutS 和 MutL 的化学计量学表明了一种修复机制。
Nucleic Acids Res. 2012 May;40(9):3929-38. doi: 10.1093/nar/gkr1298. Epub 2012 Jan 12.
7
Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion.修饰碱基通过错配修复逃避实现高效的寡核苷酸介导的等位基因替换。
Nucleic Acids Res. 2011 Sep 1;39(16):7336-47. doi: 10.1093/nar/gkr183. Epub 2011 May 23.
8
Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.错配修复突变体中λ Red介导的重组体水平增强。
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15748-53. doi: 10.1073/pnas.2434959100. Epub 2003 Dec 12.
本文引用的文献
1
Saturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coli.大肠杆菌中一种碱基类似物导致的DNA错配修复饱和与错误灾难
Genetics. 2002 Aug;161(4):1363-71. doi: 10.1093/genetics/161.4.1363.
2
Amplification of mutator cells in a population as a result of horizontal transfer.由于水平转移导致群体中突变细胞的扩增。
J Bacteriol. 2001 Jun;183(12):3737-41. doi: 10.1128/JB.183.12.3737-3741.2001.
3
Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation.细菌进化中的二阶选择：适应过程中作用于突变和重组率的选择
Res Microbiol. 2001 Jan-Feb;152(1):11-6. doi: 10.1016/s0923-2508(00)01163-3.
4
Evolutionary implications of the frequent horizontal transfer of mismatch repair genes.错配修复基因频繁水平转移的进化意义
Cell. 2000 Nov 22;103(5):711-21. doi: 10.1016/s0092-8674(00)00175-6.
5
Interspecies recombination and mismatch repair. Generation of mosaic genes and genomes.种间重组与错配修复。镶嵌基因和基因组的产生。
Methods Mol Biol. 2000;152:149-57. doi: 10.1385/1-59259-068-3:149.
6
Molecular keys to speciation: DNA polymorphism and the control of genetic exchange in enterobacteria.物种形成的分子关键：肠道细菌中的DNA多态性与基因交换控制
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9763-7. doi: 10.1073/pnas.94.18.9763.
7
Proliferation of mutators in A cell population.A细胞群体中突变体的增殖。
J Bacteriol. 1997 Jan;179(2):417-22. doi: 10.1128/jb.179.2.417-422.1997.
8
Multicopy single-stranded DNA of Escherichia coli enhances mutation and recombination frequencies by titrating MutS protein.大肠杆菌的多拷贝单链DNA通过滴定MutS蛋白提高突变和重组频率。
Mol Microbiol. 1996 Feb;19(3):505-9. doi: 10.1046/j.1365-2958.1996.392921.x.
9
Genetic barriers among bacteria.细菌之间的遗传屏障
Trends Microbiol. 1996 Feb;4(2):69-72. doi: 10.1016/0966-842X(96)81514-9.
10
Homologous recombination between the tuf genes of Salmonella typhimurium.鼠伤寒沙门氏菌tuf基因之间的同源重组。
J Mol Biol. 1996 Jul 26;260(4):506-22. doi: 10.1006/jmbi.1996.0418.
Zotero 插件