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2-氨基嘌呤通过可逆性失活大肠杆菌错配修复系统实现种间重组。

2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.

作者信息

Matic Ivan, Babic Ana, Radman Miroslav

机构信息

INSERM U571, Faculté de Médecine, Necker-Enfants Malades, Université René Descartes, 75730 Paris Cedex 15, France.

出版信息

J Bacteriol. 2003 Feb;185(4):1459-61. doi: 10.1128/JB.185.4.1459-1461.2003.

DOI:10.1128/JB.185.4.1459-1461.2003
PMID:12562818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC142871/
Abstract

2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.

摘要

用2-氨基嘌呤处理大肠杆菌可诱导出DNA错配修复缺陷的可逆表型。这种短暂的表型导致与肠炎沙门氏菌鼠伤寒血清型Hfr供体的种间接合重组频率增加300倍。该方法可通过允许分化的基因和基因组之间进行重组来用于生物多样性的产生。

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2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.2-氨基嘌呤通过可逆性失活大肠杆菌错配修复系统实现种间重组。
J Bacteriol. 2003 Feb;185(4):1459-61. doi: 10.1128/JB.185.4.1459-1461.2003.
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Mechanism and control of interspecies recombination in Escherichia coli. I. Mismatch repair, methylation, recombination and replication functions.大肠杆菌种间重组的机制与调控。I.错配修复、甲基化、重组与复制功能。
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Saturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coli.大肠杆菌中一种碱基类似物导致的DNA错配修复饱和与错误灾难
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Evolutionary implications of the frequent horizontal transfer of mismatch repair genes.错配修复基因频繁水平转移的进化意义
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Interspecies recombination and mismatch repair. Generation of mosaic genes and genomes.种间重组与错配修复。镶嵌基因和基因组的产生。
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Multicopy single-stranded DNA of Escherichia coli enhances mutation and recombination frequencies by titrating MutS protein.大肠杆菌的多拷贝单链DNA通过滴定MutS蛋白提高突变和重组频率。
Mol Microbiol. 1996 Feb;19(3):505-9. doi: 10.1046/j.1365-2958.1996.392921.x.
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Genetic barriers among bacteria.细菌之间的遗传屏障
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