2-氨基嘌呤通过可逆性失活大肠杆菌错配修复系统实现种间重组。
2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system.
作者信息
Matic Ivan, Babic Ana, Radman Miroslav
机构信息
INSERM U571, Faculté de Médecine, Necker-Enfants Malades, Université René Descartes, 75730 Paris Cedex 15, France.
出版信息
J Bacteriol. 2003 Feb;185(4):1459-61. doi: 10.1128/JB.185.4.1459-1461.2003.
Abstract
2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.
摘要
用2-氨基嘌呤处理大肠杆菌可诱导出DNA错配修复缺陷的可逆表型。这种短暂的表型导致与肠炎沙门氏菌鼠伤寒血清型Hfr供体的种间接合重组频率增加300倍。该方法可通过允许分化的基因和基因组之间进行重组来用于生物多样性的产生。
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