重组工程与多重自动基因组工程技术
Recombineering and MAGE.
作者信息
Wannier Timothy M, Ciaccia Peter N, Ellington Andrew D, Filsinger Gabriel T, Isaacs Farren J, Javanmardi Kamyab, Jones Michaela A, Kunjapur Aditya M, Nyerges Akos, Pal Csaba, Schubert Max G, Church George M
机构信息
Department of Genetics, Harvard Medical School, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
出版信息
Nat Rev Methods Primers. 2021;1. doi: 10.1038/s43586-020-00006-x. Epub 2021 Jan 14.
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved genome engineering capabilities, perhaps none more so than multiplex automated genome engineering (MAGE). MAGE is representative of a set of highly multiplexed single-stranded DNA-mediated technologies. First described in , both MAGE and recombineering are being rapidly translated into diverse prokaryotes and even into eukaryotic cells. Together, this modern set of tools offers the promise of radically improving the scope and throughput of experimental biology by providing powerful new methods to ease the genetic manipulation of model and non-model organisms. In this Primer, we describe recombineering and MAGE, their optimal use, their diverse applications and methods for pairing them with other genetic editing tools. We then look forward to the future of genetic engineering.
重组介导的基因工程，也称为重组工程，是将同源单链或双链DNA整合到细菌基因组中。重组工程及其衍生方法极大地提高了基因组工程能力，其中多重自动化基因组工程（MAGE）可能尤为突出。MAGE是一组高度多重化的单链DNA介导技术的代表。MAGE和重组工程最早在[具体时间]被描述，目前正迅速应用于多种原核生物甚至真核细胞。这一套现代工具共同提供了通过提供强大的新方法来简化模式生物和非模式生物的基因操作，从而从根本上扩大实验生物学的范围并提高其通量的前景。在本入门指南中，我们描述了重组工程和MAGE、它们的最佳使用方法、它们的各种应用以及将它们与其他基因编辑工具配对的方法。然后我们展望基因工程的未来。
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