Moreau S, Prensier G, Maalla J, Fortier B
Eur J Cell Biol. 1986 Dec;42(2):207-10.
We report the synthesis of an analogue of chloroquine (CQA) which can be used as a probe to visualize accumulation of 4-aminoquinoline by electron microscopy. A mouse monoclonal antibody against CQA was raised and used for immunodetection by the protein-A gold method on ultrathin cryosections, of CQA treated parasites. We demonstrate that in a P. berghei chloroquine(CQ)-sensitive strain (N strain) the chloroquine analogue used accumulates in the endocytic vacuoles where hemoglobin (Hb) degradation is occurring. In contrast, in a P. berghei CQ-resistant strain (RC strain) the probe was found scattered all over the cytoplasm of the parasite. This result suggests that endocytic vacuoles of the parasite could constitute the site of antimalarial action of CQ.
我们报道了一种氯喹类似物（CQA）的合成，该类似物可用作探针，通过电子显微镜观察4-氨基喹啉的积累情况。制备了一种抗CQA的小鼠单克隆抗体，并通过蛋白A金标法对经CQA处理的寄生虫超薄冷冻切片进行免疫检测。我们证明，在伯氏疟原虫氯喹（CQ）敏感株（N株）中，所使用的氯喹类似物积累在内吞液泡中，血红蛋白（Hb）降解正在此发生。相反，在伯氏疟原虫CQ耐药株（RC株）中，发现探针分散在寄生虫的整个细胞质中。这一结果表明，寄生虫的内吞液泡可能是CQ抗疟作用的位点。
