Cheng Y, Zhang Y, Lin C, Zhan Z, Yu X, Lin J, Cai M, Che W
Fujian Provincial Institute of Parasitic Discases, Fuzhou 350001.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(3):135-9.
To explore the identification and differentiation of Echinochasmus fujianensis, Echinochasmus japonicus(Jiangxi strain and Fujian strain), Echinochasmus liliputanus Anhui and Echinochasmus per foliatus Hubei.
Random amplified polymorphic DNA analysis (RAPD) and experimental animal infection were performed.
469 polymorphic DNA fragments were obtained by 28 primers from 4 Echinochasmus species and strains in Fujian, Anhui and Jiangxi. 20.8% and 97.6% of the fragments in Echinochasmus fujianensis were the same as those in Echinochasmus japonicus Fujian strain and in Echinochasmus liliputanus Anhui, respectively, 99.7% of the fragments were the same between Echinochasmus japonicus Jiangxi strain and Fujian strain.
Echinochasmus fujianensis and Echinochasmus liliputanus Anhui are the same species. Echinochasmus fujienensis is an independent species different from Echinochasmus japonicus. Polyinfection of Echinochasmus fujianensis, Echinochasmus japonicus and Echinochasmus perfoliatus exist in all the 3 provinces, Hubei, Anbui and Fujian, of which Echinochasmus fujienensis is a dominant species.
探索福建棘隙吸虫、日本棘隙吸虫（江西株和福建株）、安徽微小棘隙吸虫和湖北叶状棘隙吸虫的鉴别与区分。
进行随机扩增多态性DNA分析（RAPD）及实验动物感染。
用28条引物对福建、安徽和江西的4种棘隙吸虫虫种及虫株进行RAPD分析，共获得469条多态性DNA片段。福建棘隙吸虫与日本棘隙吸虫福建株、安徽微小棘隙吸虫的片段相同率分别为20.8%和97.6%，日本棘隙吸虫江西株与福建株的片段相同率为99.7%。
福建棘隙吸虫与安徽微小棘隙吸虫为同一种，福建棘隙吸虫是不同于日本棘隙吸虫的独立虫种。湖北、安徽和福建3省均存在福建棘隙吸虫、日本棘隙吸虫和叶状棘隙吸虫的混合感染，其中福建棘隙吸虫为优势虫种。
