Ohta Mitsuhiro, Ohta Kiyoe
Department of Clinical Chemistry, Kobe Pharmaceutical University, Higashinadu-ku, Kobe 658-8558, Japan.
Expert Rev Mol Diagn. 2002 Nov;2(6):627-33. doi: 10.1586/14737159.2.6.627.
Radioimmunoassay for myelin basic protein in cerebrospinal fluid is commonly used as a biochemical marker of demyelination in multiple sclerosis patients. A sensitive enzyme-linked immunosorbent assay for myelin basic protein has been recently developed, which can make a clinical evaluation of myelin basic protein in cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases. Most multiple sclerosis patients with acute exacerbation had markedly high myelin basic protein. Longitudinal studies of multiple sclerosis patients showed that myelin basic protein in CSF increases rapidly in agreement with acute relapse and then rapidly declines and disappears. Significantly higher cerebrospinal fluid myelin basic protein levels in human T-cell lymphotropic virus Type I-associated myelopathy/tropical spastic paraparesis patients were also detected. This enzyme-linked immunosorbent assay system can be used routinely to measure myelin basic protein in cerebrospinal fluid as a useful diagnostic indicator, not only for central active demyelination as in multiple sclerosis but, also for spinal cord demyelination as in human T-cell lymphotropic virus Type I-associated myelopathy/tropical spastic paraparesis.
脑脊液中髓鞘碱性蛋白的放射免疫测定通常用作多发性硬化症患者脱髓鞘的生化标志物。最近开发了一种灵敏的髓鞘碱性蛋白酶联免疫吸附测定法，可对多发性硬化症和其他神经系统疾病患者脑脊液中的髓鞘碱性蛋白进行临床评估。大多数急性加重期的多发性硬化症患者的髓鞘碱性蛋白明显升高。对多发性硬化症患者的纵向研究表明，脑脊液中的髓鞘碱性蛋白会随着急性复发迅速增加，然后迅速下降并消失。在人类嗜T细胞病毒I型相关脊髓病/热带痉挛性截瘫患者中也检测到脑脊液髓鞘碱性蛋白水平显著更高。这种酶联免疫吸附测定系统可常规用于测量脑脊液中的髓鞘碱性蛋白，作为一种有用的诊断指标，不仅用于多发性硬化症中的中枢性活动性脱髓鞘，也用于人类嗜T细胞病毒I型相关脊髓病/热带痉挛性截瘫中的脊髓脱髓鞘。
