Sasaki Atsushi, Horikoshi Yuko, Yokoo Hideaki, Nakazato Yoichi, Yamaguchi Haruyasu
Department of Pathology, Gunma University School of Medicine, Maebashi 371-8511, Japan.
Neurosci Lett. 2003 Feb 20;338(1):17-20. doi: 10.1016/s0304-3940(02)01332-0.
Human monocytes and a variety of tissue macrophages, including microglia, were studied immunohistochemically to determine the expression of a novel microglial marker, human glucose transporter 5 (hGLUT5), in these cells. The hGLUT5 was not expressed in most peripheral macrophages in the normal state, but weakly expressed in some foamy macrophages in atherosclerotic lesions. There was no hGLUT5 reactivity in blood monocytes. In the lesions of brain infarcts, foamy macrophages (predominantly monocyte-derived cells) in the ischemic core were mostly negative for hGLUT5, while activated and phagocytic microglia in the transitional zone were consistently positive. The present study indicated that unlike other microglial markers, hGLUT5 is rarely present in peripheral macrophages, and that hGLUT5 immunohistochemistry is useful in distinguishing microglia-derived macrophages from monocyte-derived macrophages in acute necrotic lesions.
为了确定一种新型小胶质细胞标志物——人葡萄糖转运蛋白5（hGLUT5）在这些细胞中的表达情况，我们采用免疫组织化学方法对人单核细胞和包括小胶质细胞在内的多种组织巨噬细胞进行了研究。在正常状态下，大多数外周巨噬细胞不表达hGLUT5，但在动脉粥样硬化病变中的一些泡沫巨噬细胞中呈弱表达。血液单核细胞中无hGLUT5反应性。在脑梗死病变中，缺血核心区的泡沫巨噬细胞（主要是单核细胞衍生细胞）大多hGLUT5呈阴性，而过渡区活化的吞噬性小胶质细胞则始终呈阳性。本研究表明，与其他小胶质细胞标志物不同，hGLUT5在外周巨噬细胞中很少存在，并且hGLUT5免疫组织化学有助于在急性坏死性病变中区分小胶质细胞衍生的巨噬细胞和单核细胞衍生的巨噬细胞。
