Kingsmore Stephen F, Patel Dhavalkumar D
Molecular Staging Inc., 300 George Street, New Haven, CT 06511, USA.
Curr Opin Biotechnol. 2003 Feb;14(1):74-81. doi: 10.1016/s0958-1669(02)00019-8.
Multiplexed immunoassays on antibody-based protein microarrays are an attractive solution for analyzing biological responses in normal and diseased states. Recently, the feasibility and utility of these assays has been established as concerns about specificity and sensitivity are being overcome by careful quality control and amplification technologies such as rolling circle amplification (RCA). RCA-amplified protein chips can now profile up to 150 proteins in various substrates including serum, plasma, and supernatants with high sensitivity, broad dynamic range and good reproducibility. Diagnostic utility of RCA-amplified protein chips has been shown for multiplexed allergen testing. When allied with multivariate statistical analysis, RCA protein chips have the potential to identify multiplexed biomarker classifiers for disease diagnosis and drug response.
基于抗体的蛋白质微阵列上的多重免疫测定是分析正常和疾病状态下生物反应的一种有吸引力的解决方案。最近，随着通过诸如滚环扩增（RCA）等仔细的质量控制和扩增技术克服了对特异性和灵敏度的担忧，这些测定的可行性和实用性已得到确立。RCA扩增的蛋白质芯片现在可以在包括血清、血浆和上清液在内的各种底物中以高灵敏度、宽动态范围和良好的重现性分析多达150种蛋白质。RCA扩增的蛋白质芯片在多重过敏原检测中的诊断效用已得到证明。当与多变量统计分析相结合时，RCA蛋白质芯片有潜力识别用于疾病诊断和药物反应的多重生物标志物分类器。
