锥虫蓝在体外对视网膜色素上皮无毒。
Trypan blue not toxic for retinal pigment epithelium in vitro.
作者信息
Stalmans Peter, Van Aken Elisabeth H, Melles Gerrit, Veckeneer Marc, Feron Eric J, Stalmans Ingeborg
机构信息
Department of Ophthalmology, UZLeuven, Leuven, Belgium.
出版信息
Am J Ophthalmol. 2003 Feb;135(2):234-6. doi: 10.1016/s0002-9394(02)01891-3.
PURPOSE
To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.
DESIGN
Experimental study with a direct live/dead cell staining technique using fluorescent dyes.
METHODS
Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.
RESULTS
No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.
CONCLUSION
These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.
目的
研究台盼蓝对培养的视网膜色素上皮（RPE）细胞是否具有毒性作用。
设计
采用荧光染料进行直接活/死细胞染色技术的实验研究。
方法
将培养的人视网膜色素上皮细胞暴露于不同浓度的台盼蓝（0.06%、0.15%、0.30%）中5分钟，并用共聚焦显微镜测量细胞活力。
结果
在所使用的任何台盼蓝浓度下培养的细胞中，均未发现细胞死亡增加。
结论
这些发现表明，短时间暴露于台盼蓝对培养的视网膜色素上皮细胞没有毒性作用。
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