Stalmans Peter, Van Aken Elisabeth H, Veckeneer Marc, Feron Eric J, Stalmans Ingeborg
Department of Ophthalmology UZ Leuven, Leuven, Belgium.
Am J Ophthalmol. 2002 Aug;134(2):282-5. doi: 10.1016/s0002-9394(02)01468-x.
To investigate whether the toxic effect on cultured retinal pigment epithelial (RPE) cells after application of indocyanine green is related to the osmolarity of the solvent or to toxic effects of the dye and evaluate whether these changes also occur using infracyanine green.
Experimental study with a direct live/dead cell staining technique using fluorescent dyes.
Cultured human RPE cells were exposed to various solutions and cell viability was confocally measured.
Increased cell death was found in cultures incubated in the hypoosmotic solvent that is generally used for indocyanine green (P <.001, n = 12). Addition of indocyanine green did not alter this observation (P <.001, n = 12). In cultures exposed to a 5% glucose solution, no increased cell death was found (P =.94, n = 12), nor when infracyanine green was added (P =.13, n = 12).
The observed toxicity of indocyanine green on RPE cells is probably related to the hypo-osmolarity of the solvent and may be avoided by using infracyanine green dissolved in glucose 5%.
研究应用吲哚菁绿后对培养的视网膜色素上皮（RPE）细胞的毒性作用是否与溶剂的渗透压有关，还是与染料的毒性作用有关，并评估使用亚甲蓝绿时是否也会发生这些变化。
采用荧光染料直接进行活/死细胞染色技术的实验研究。
将培养的人RPE细胞暴露于各种溶液中，并通过共聚焦显微镜测量细胞活力。
在通常用于吲哚菁绿的低渗溶剂中培养的细胞中发现细胞死亡增加（P<.001，n = 12）。添加吲哚菁绿并未改变这一观察结果（P<.001，n = 12）。在暴露于5%葡萄糖溶液的培养物中，未发现细胞死亡增加（P =.94，n = 12），添加亚甲蓝绿时也未发现（P =.13，n = 12）。
观察到的吲哚菁绿对RPE细胞的毒性可能与溶剂的低渗性有关，使用溶解于5%葡萄糖中的亚甲蓝绿可能避免这种毒性。
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