Han X L, Liu D Q, Feng X P
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(1):8-10.
To develop an in vitro method for the assessment of drug response in P. falciparum to pyronaridine/sulfadoxine/pyrimethamine (PND/S/P).
The PND/S/P microtest plate was designed, Rieckmann in vitro microtest(WHO standard kit) was used to test the sensitivity of P falciparum in continuous culture (FCC1/HN strain), and the data obtained were analyzed using a computer programme. Fractional inhibitory concentration (FIC) was calculated to test the possible synergy between PND and S/P.
The effect of the PND/S/P plates was fairly stable and the ED50 values of pyronaridine, sulfadoxine and pyrimethamine were 0.11, 215.12 and 2.9 pmol, respectively. The FIC obtained confirmed the synergism between PND and S/P.
The in vitro method can be used to assess the sensitivity of P. falciparum to pyronaridine/sulfadoxine/pyrimethamine.
建立一种体外评估恶性疟原虫对咯萘啶/磺胺多辛/乙胺嘧啶（PND/S/P）药物反应的方法。
设计PND/S/P微量测试板，采用里克曼体外微量测试法（WHO标准试剂盒）检测连续培养的恶性疟原虫（FCC1/HN株）的敏感性，并使用计算机程序分析所得数据。计算分数抑制浓度（FIC）以检测PND与S/P之间可能的协同作用。
PND/S/P板的效果相当稳定，咯萘啶、磺胺多辛和乙胺嘧啶的半数有效剂量（ED50）值分别为0.11、215.12和2.9皮摩尔。获得的FIC证实了PND与S/P之间的协同作用。
该体外方法可用于评估恶性疟原虫对咯萘啶/磺胺多辛/乙胺嘧啶的敏感性。
