[通过表达序列标签从日本血吸虫中获取并构建新基因]
[Obtaining and construction of a novel gene from Schistosoma japonicum by expressed sequence tags].
作者信息
Li Yan, Yu Xin-bing, Wu Zhong-dao, Li Hui-ting, Bao Jun-ying, Shao Xiao
机构信息
Department of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(1):32-4.
OBJECTIVE
To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum.
METHODS
New genes of S. japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector.
RESULTS
An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3.
CONCLUSION
The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene's function.
目的
寻找日本血吸虫新的候选疫苗分子并对新基因进行特性分析。
方法
通过表达序列标签获得日本血吸虫新基因并进行在线分析。将一个感兴趣的cDNA克隆到反义真核表达载体中。
结果
获得一个感兴趣的cDNA JAYL0230。分析表明其推导的氨基酸序列与人、猪等的抗凋亡死亡因子1存在同源性。将其反义cDNA克隆到真核表达载体pEGFP-N3中。
结论
表达序列标签与生物信息学相结合是发现生物新基因的有效方法。构建反义真核载体有助于研究基因功能。
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