Bian Guowu, Yu Xinbing, Wu Zhongdao
The Department of Parasitology, Sun Yet-sen University of Medical Sciences, Guangzhou 510089, China.
Chin Med J (Engl). 2002 Oct;115(10):1517-20.
To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST).
A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ESTs). These were compared against an EMBL-parasites database and GENBANK database by BLASTn and BLASTx.
A total of 314 phage clones were randomly selected for generating expressed sequence tags (ESTs). From these clones, 132 EST-quality sequence were obtained. Among these EST-quality sequences, 113 ESTs were successfully submitted to the dbEST at GenBanK. A total of 7.6% of these EST-quality sequences were previously identified sequence of Schistosoma japonicum, while 4.5% were putatively identified sequences of Schistosoma japonicum. A total of 23.5% of these EST-quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms. 57.6% had no matches in the database and were classified as unknown sequences. Most ESTs with the putative protein identified belonged to housekeeping proteins. Information about several interesting genes was found.
Partial cDNA sequencing to generate expressed sequence tags (ESTs) has the potential to rapidly and economically increase our knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes.
利用表达序列标签（EST）快速且经济地获取日本血吸虫（中国株）成虫期表达基因的相关知识。
使用从日本血吸虫（中国株）成虫期RNA构建的定向cDNA文库来生成表达序列标签（EST）。通过BLASTn和BLASTx将这些标签与EMBL寄生虫数据库和GENBANK数据库进行比对。
总共随机选择314个噬菌体克隆来生成表达序列标签（EST）。从这些克隆中，获得了132条EST质量序列。在这些EST质量序列中，113条EST成功提交至GenBanK的dbEST。这些EST质量序列中，7.6%是先前已鉴定的日本血吸虫序列，4.5%是推测鉴定的日本血吸虫序列。这些EST质量序列中，总共23.5%是推测鉴定的曼氏血吸虫或其他生物的序列。57.6%在数据库中无匹配，被归类为未知序列。大多数推测鉴定出蛋白质的EST属于管家蛋白。发现了一些有趣基因的信息。
通过部分cDNA测序生成表达序列标签（EST）有潜力快速且经济地增加我们对日本血吸虫（中国株）成虫期表达基因的了解。
