[中国日本血吸虫不同分离株的基因组多样性研究]

[Study on genomic diversity among different isolates of Schistosoma japonicum in China].

作者信息

Qiu Chi-ping, Chris Spolsky, Xia Ming-yi, Chen Qin, Tong Xiao-mei, Gong Xin, Davis George, Feng Zheng

机构信息

Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(2):79-82.

PMID:12567563

Abstract

OBJECTIVE

To study genomic diversity among the isolates of Schistosoma japonicum in China.

METHODS

Schistosome adults were collected from different endemic areas. Mitochondrial NADH dehydrogenase 1 (ND1) and cytochrome C oxidase 1 (CO1) gene fragments of the worms were amplified by PCR, cloned into plasmid, and finally sequenced. Program MEGA was used for sequence analysis.

RESULTS

The ND1 and CO1 genes amplified from different geographic strains were 476 bp and 1,033 bp, respectively. There are two distinct haplotypes, type I and II, for both ND1 and CO1 nucleotide sequences. The differences between the two types were 4.0% and 3.4%. They are considered to be two different genetypes by the phylogenetic analysis. In individual mitochondrial gene, type I of ND1 was fixedly accompanied with type I of CO1 gene, and type II of ND1 with type II of CO1.

CONCLUSION

There are two different genetypes of ND1 and CO1 genes of S. japonicum in China.

摘要

目的

研究中国日本血吸虫分离株之间的基因组多样性。

方法

从不同流行地区收集血吸虫成虫。通过聚合酶链反应（PCR）扩增虫体的线粒体烟酰胺腺嘌呤二核苷酸脱氢酶1（ND1）和细胞色素C氧化酶1（CO1）基因片段，克隆到质粒中，最后进行测序。使用MEGA程序进行序列分析。

结果

从不同地理株扩增得到的ND1和CO1基因分别为476 bp和1033 bp。ND1和CO1核苷酸序列均有两种不同的单倍型，即I型和II型。两种类型之间的差异分别为4.0%和3.4%。通过系统发育分析，它们被认为是两种不同的基因型。在单个线粒体基因中，ND1的I型与CO1基因的I型固定相伴，ND1的II型与CO1的II型相伴。