In vitro antimalarial effect of daphnetin relating to its iron-chelating activity.
作者信息
Mu Ling-yun, Wang Qin-mei, Ni Yi-chang
机构信息
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(2):83-5.
OBJECTIVE
To investigate the in vitro antimalarial effect of daphentin relating to its iron-chelating activity.
METHODS
Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein.
RESULTS
Daphnetin exhibited a modest iron-chelating ability compared with the powerful iron-chelator desferrioxamine B. In vitro test at the range of 0-12 mumol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe2+ in a ratio of 2:1.
CONCLUSION
The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.
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