Mu Ling-yun, Wang Qin-mei, Ni Yi-chang
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(2):83-5.
To investigate the in vitro antimalarial effect of daphentin relating to its iron-chelating activity.
Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein.
Daphnetin exhibited a modest iron-chelating ability compared with the powerful iron-chelator desferrioxamine B. In vitro test at the range of 0-12 mumol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe2+ in a ratio of 2:1.
The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.
