[瑞香素对恶性疟原虫超氧化物歧化酶活性及体外DNA合成的影响]
[Effect of daphnetin on SOD activity and DNA synthesis of Plasmodium falciparum in vitro].
作者信息
Mu Ling-yun, Wang Qin-mei, Ni Yi-chang
机构信息
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(3):157-9.
OBJECTIVE
To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro.
METHODS
The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P.f) FCC1 in vitro was determined with a SOD test-kit. The level of DNA synthesis of P.f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258.
RESULTS
The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P.f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P.f trophozoites in synchronized in vitro culture was significantly lower than that of the control.
CONCLUSION
Daphnetin lowered SOD activity and decreased DNA synthesis of P.f in vitro.
目的
研究瑞香素对恶性疟原虫超氧化物歧化酶（SOD）活性及DNA合成的体外影响。
方法
用SOD检测试剂盒测定瑞香素、瑞香素 - 铁复合物和去铁胺B对体外培养的恶性疟原虫（P.f）FCC1株SOD活性的影响。用荧光染料Hoechest 33258检测瑞香素或去铁胺B处理后体外同步培养的处于不同发育阶段的恶性疟原虫的DNA合成水平。
结果
瑞香素处理后总SOD活性降低60%，而去铁胺B处理后仅降低22%。未观察到瑞香素 - 铁复合物对恶性疟原虫SOD活性有影响。体外同步培养的恶性疟原虫滋养体的DNA合成水平显著低于对照组。
结论
瑞香素可降低恶性疟原虫的SOD活性并减少其体外DNA合成。
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