Brown S E, Gorman B M, Tesh R B, Knudson D L
Department of Entomology, College of Agricultural Sciences, Colorado State University, Fort Collins 80523.
Virology. 1993 Sep;196(1):363-7. doi: 10.1006/viro.1993.1490.
Viruses with genomes consisting of 12 segments of double-stranded (ds) RNA were isolated from mosquito pools collected in Indonesia in 1980 and 1981. The genome segments of 11 virus isolates exhibited two distinct electrophoretic patterns in agarose gels, and the viruses probably represent distinct groups of Coltiviruses. Ten of the 11 virus isolates produced recognizably similar PAGE profiles of two groups of 6 segments (6-6) with 3 distinct profile variants, and the remaining virus isolate displayed a unique 6-5-1 profile. These profiles were distinct from that of Colorado tick fever (CTF) virus RNA. The genetic relatedness of the isolates was examined by RNA-RNA hybridization. The viruses with a 6-6 profile were closely related in the majority of their segments, with segments 7, 11, and 12 being variant; and segment 9 the most divergent. The segments of the virus with the unique PAGE profile (6-5-1) did not cross-hybridize with any of the other viruses. Similarly, RNA from virus isolates from Indonesian did not cross-hybridize with the segments of CTF virus. These data indicate that two additional genotypes are present in the Coltivirus genus.
1980年和1981年从印度尼西亚采集的蚊虫样本中分离出了基因组由12条双链（ds）RNA片段组成的病毒。11株病毒分离株的基因组片段在琼脂糖凝胶中呈现出两种不同的电泳图谱，这些病毒可能代表不同的科罗拉多蜱传热病毒群。11株病毒分离株中的10株产生了两组6个片段（6-6）的明显相似的聚丙烯酰胺凝胶电泳图谱，有3种不同的图谱变体，其余病毒分离株呈现独特的6-5-1图谱。这些图谱与科罗拉多蜱传热（CTF）病毒RNA的图谱不同。通过RNA-RNA杂交检测了分离株之间的遗传相关性。具有6-6图谱的病毒在其大多数片段上密切相关，片段7、11和12为变体；片段9差异最大。具有独特聚丙烯酰胺凝胶电泳图谱（6-5-1）的病毒片段与其他任何病毒均未发生交叉杂交。同样，来自印度尼西亚的病毒分离株的RNA与CTF病毒的片段也未发生交叉杂交。这些数据表明科罗拉多蜱传热病毒属中还存在另外两种基因型。
