Zhu X P, Zhou L, Zhang X M, Yang J, Yang Y P
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2001;19(1):1-3.
To determine the genotypes and distribution of MSP2 of Plasmodium falciparum isolates in Yunnan and Hainan provinces, China.
The central polymorphic region of MSP2 allele was amplified by the nested PCR for genotyping of P. falciparum.
The higher degree of polymorphism of MSP2 of P. falciparum was observed in Yunnan and Hainan. Distribution and allele frequencies differed in both provinces, indicating considerable geographical heterogeneity of parasite populations. The mixed infection of different allele-type and multiplicity of infection was more frequent in Hainan than in Yunnan.
There were obvious differences in the distribution and frequencies of MSP2 alleles between Yunnan and Hainan endemic areas. MSP2 is suitable to be used as a marker gene for the genotyping of P. falciparum infection.
确定中国云南省和海南省恶性疟原虫分离株MSP2的基因型及分布情况。
采用巢式PCR扩增MSP2等位基因的中央多态性区域，对恶性疟原虫进行基因分型。
在云南和海南观察到恶性疟原虫MSP2具有较高的多态性程度。两省的分布及等位基因频率存在差异，表明疟原虫种群存在显著的地理异质性。海南不同等位基因类型的混合感染及感染复数比云南更常见。
云南和海南疟疾流行区MSP2等位基因的分布和频率存在明显差异。MSP2适合用作恶性疟原虫感染基因分型的标记基因。
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