用于测定肺炎链球菌血清型和血清群的半自动多重聚合酶链反应检测方法的评估
Evaluation of semiautomated multiplex PCR assay for determination of Streptococcus pneumoniae serotypes and serogroups.
作者信息
Lawrence Elliot R, Griffiths David B, Martin Siobhán A, George Robert C, Hall Lucinda M C
机构信息
Department of Medical Microbiology, Barts and The London School of Medicine and Dentistry, Queen Mary, University of London, London E1 2AD.
出版信息
J Clin Microbiol. 2003 Feb;41(2):601-7. doi: 10.1128/JCM.41.2.601-607.2003.
Abstract
A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresis with a 7-min run time, and a serotype or serogroup was assigned on the basis of fragment size. The method was used to test 93 clinical isolates, and all isolates of the serotypes concerned were correctly detected. The strategy would allow the detection of multiple serotypes in a single sample. Detection of additional serotypes could be included as capsule locus sequences become available.
摘要
开发了一种用于测定肺炎链球菌中五种血清型和三个血清群的半自动方法。针对血清型1、3、14、19F和23F以及血清群6、19和23的特异性引物被组合在三个多重PCR中。产物通过运行时间为7分钟的毛细管电泳进行分离，并根据片段大小确定血清型或血清群。该方法用于检测93株临床分离株，所有相关血清型的分离株均被正确检测。该策略可在单个样本中检测多种血清型。随着更多荚膜基因座序列的获得，可纳入对其他血清型的检测。
相似文献
1
Evaluation of semiautomated multiplex PCR assay for determination of Streptococcus pneumoniae serotypes and serogroups.用于测定肺炎链球菌血清型和血清群的半自动多重聚合酶链反应检测方法的评估
J Clin Microbiol. 2003 Feb;41(2):601-7. doi: 10.1128/JCM.41.2.601-607.2003.
2
Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China.在中国深圳，通过多重连接依赖探针扩增和熔解曲线（MLPA-MC）分析法在一次聚合酶链反应中对十种常见肺炎链球菌血清型/血清群进行实际预测。
PLoS One. 2015 Jul 7;10(7):e0130664. doi: 10.1371/journal.pone.0130664. eCollection 2015.
3
PCR-based assays for detection of Streptococcus pneumoniae serotypes 3, 14, 19F and 23F in respiratory specimens.用于检测呼吸道标本中肺炎链球菌3型、14型、19F型和23F型血清型的基于聚合酶链反应的检测方法。
J Med Microbiol. 2004 Jul;53(Pt 7):595-602. doi: 10.1099/jmm.0.45550-0.
4
PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18.基于PCR的肺炎链球菌培养阴性标本血清分型：用于检测18血清群内血清型的新型引物
J Clin Microbiol. 2016 Aug;54(8):2178-81. doi: 10.1128/JCM.00419-16. Epub 2016 Jun 1.
5
Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolates.用于确定肺炎链球菌分离株血清型或血清群的新型聚合酶链反应-限制性片段长度多态性方法。
J Clin Microbiol. 2005 Jun;43(6):2656-61. doi: 10.1128/JCM.43.6.2656-2661.2005.
6
[Establishment and application of PCR method in serotyping of Streptococcus pneumonia.].
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Mar;31(3):316-20.
7
A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR.一种利用部分cpsA-cpsB测序以及wzy或wzx特异性PCR对90种肺炎链球菌血清型进行分子荚膜型预测的系统。
J Med Microbiol. 2005 Apr;54(Pt 4):351-356. doi: 10.1099/jmm.0.45924-0.
8
PCR-based discrimination of emerging Streptococcus pneumoniae serotypes 22F and 33F.基于聚合酶链反应（PCR）对新出现的肺炎链球菌22F和33F血清型的鉴别
J Microbiol Methods. 2018 Jan;144:99-106. doi: 10.1016/j.mimet.2017.11.017. Epub 2017 Nov 21.
9
Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates.用于确定肺炎链球菌分离株荚膜血清型的序列多重聚合酶链反应方法。
J Clin Microbiol. 2006 Jan;44(1):124-31. doi: 10.1128/JCM.44.1.124-131.2006.
10
Streptococcus pneumoniae type determination by multiplex polymerase chain reaction.用多重聚合酶链反应进行肺炎链球菌型别鉴定。
J Korean Med Sci. 2011 Aug;26(8):971-8. doi: 10.3346/jkms.2011.26.8.971. Epub 2011 Jul 27.
引用本文的文献
1
Highly Resistant Serotype 19A Streptococcus pneumoniae of the GPSC1/CC320 Clone from Invasive Infections in Poland Prior to Antipneumococcal Vaccination of Children.在儿童接种抗肺炎球菌疫苗之前，波兰侵袭性感染中源自GPSC1/CC320克隆的高度耐药19A血清型肺炎链球菌。
Infect Dis Ther. 2023 Aug;12(8):2017-2037. doi: 10.1007/s40121-023-00842-w. Epub 2023 Jul 13.
2
The Molecular Approaches and Challenges of Serotyping for Epidemiological Surveillance in the Vaccine Era.分子方法在疫苗时代进行流行病学监测的血清分型的应用及挑战。
Pol J Microbiol. 2023 Jun 14;72(2):103-115. doi: 10.33073/pjm-2023-023. eCollection 2023 Jun 1.
3
Optimization of a high-throughput nanofluidic real-time PCR to detect and quantify of 15 bacterial species and 92 Streptococcus pneumoniae serotypes.高通量纳米流控实时 PCR 优化检测和定量 15 种细菌物种和 92 种肺炎链球菌血清型。
Sci Rep. 2023 Mar 21;13(1):4588. doi: 10.1038/s41598-023-31820-4.
4
Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland.波兰国家免疫计划实施时，未接种疫苗儿童中的肺炎球菌携带情况。
Sci Rep. 2022 Apr 7;12(1):5858. doi: 10.1038/s41598-022-09488-z.
5
Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples.通过荚膜序列分型（CST）进行肺炎球菌血清型鉴定：一种直接在临床样本中进行血清分型的改良新方法。
Diagnostics (Basel). 2021 Dec 14;11(12):2353. doi: 10.3390/diagnostics11122353.
6
Utility of MALDI-TOF MS as a new tool for Streptococcus pneumoniae serotyping.基质辅助激光解吸电离飞行时间质谱技术（MALDI-TOF MS）作为一种新型工具在肺炎链球菌血清分型中的应用。
PLoS One. 2019 Feb 12;14(2):e0212022. doi: 10.1371/journal.pone.0212022. eCollection 2019.
7
Assessment of Postvaccine Immunity against in Patients with Asplenia, including an Analysis of Its Impact on Bacterial Flora of the Upper Respiratory Tract and Incidence of Infections.评估脾切除患者接种疫苗后的免疫效果，包括分析其对上呼吸道细菌群落和感染发生率的影响。
J Immunol Res. 2018 Dec 31;2018:1691347. doi: 10.1155/2018/1691347. eCollection 2018.
8
Development of PCRSeqTyping-a novel molecular assay for typing of .PCRSeq分型技术的开发——一种新型的分型分子检测方法 。（原文似乎不完整，翻译可能不太准确，完整准确的翻译需结合完整原文）
Pneumonia (Nathan). 2017 May 25;9:8. doi: 10.1186/s41479-017-0032-3. eCollection 2017.
9
Emergence of Neoteric Serotypes Among Multidrug Resistant Strains of Streptococcus pneumoniae Prevalent in Egypt.埃及流行的肺炎链球菌多重耐药菌株中新型血清型的出现。
Jundishapur J Microbiol. 2016 Apr 23;9(4):e30708. doi: 10.5812/jjm.30708. eCollection 2016 Apr.
10
Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.用于确定92种肺炎球菌血清型的单步多重PCR检测法
J Clin Microbiol. 2016 Aug;54(8):2197-200. doi: 10.1128/JCM.01156-16. Epub 2016 Jun 8.
本文引用的文献
1
Molecular organization of the genes required for the synthesis of type 1 capsular polysaccharide of Streptococcus pneumoniae: formation of binary encapsulated pneumococci and identification of cryptic dTDP-rhamnose biosynthesis genes.肺炎链球菌1型荚膜多糖合成所需基因的分子组织：二元包囊肺炎球菌的形成及隐蔽性胸苷二磷酸-鼠李糖生物合成基因的鉴定
Mol Microbiol. 1997 Jul;25(1):79-92. doi: 10.1046/j.1365-2958.1997.4341801.x.
2
The prevention of pneumococcal disease in children.儿童肺炎球菌疾病的预防
N Engl J Med. 2001 Oct 18;345(16):1177-83. doi: 10.1056/NEJMra010462.
3
Molecular characterization of Streptococcus pneumoniae type 4, 6B, 8, and 18C capsular polysaccharide gene clusters.4型、6B型、8型和18C型肺炎链球菌荚膜多糖基因簇的分子特征分析
Infect Immun. 2001 Mar;69(3):1244-55. doi: 10.1128/IAI.69.3.1244-1255.2001.
4
Prevention of pneumococcal disease by vaccination: does serotype replacement matter?通过接种疫苗预防肺炎球菌疾病：血清型替换重要吗？
Lancet. 2000 Oct 7;356(9237):1210-1. doi: 10.1016/S0140-6736(00)02779-3.
5
Lack of utility of serotyping multiple colonies for detection of simultaneous nasopharyngeal carriage of different pneumococcal serotypes.对多个菌落进行血清分型以检测不同肺炎球菌血清型同时存在于鼻咽部的实用性不足。
Pediatr Infect Dis J. 2000 Oct;19(10):1017-20. doi: 10.1097/00006454-200010000-00019.
6
Efficacy, safety and immunogenicity of heptavalent pneumococcal conjugate vaccine in children. Northern California Kaiser Permanente Vaccine Study Center Group.七价肺炎球菌结合疫苗在儿童中的有效性、安全性及免疫原性。北加利福尼亚凯撒医疗集团疫苗研究中心团队
Pediatr Infect Dis J. 2000 Mar;19(3):187-95. doi: 10.1097/00006454-200003000-00003.
7
Evaluation of serotype prediction by cpsA-cpsB gene polymorphism in Streptococcus pneumoniae.肺炎链球菌中cpsA-cpsB基因多态性对血清型预测的评估
J Clin Microbiol. 2000 Apr;38(4):1319-23. doi: 10.1128/JCM.38.4.1319-1323.2000.
8
Development of a PCR assay for rapid detection of enterococci.用于快速检测肠球菌的聚合酶链反应检测方法的开发。
J Clin Microbiol. 1999 Nov;37(11):3497-503. doi: 10.1128/JCM.37.11.3497-3503.1999.
9
Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types belonging to serogroup 19.19血清群肺炎链球菌荚膜多糖生物合成的比较遗传学
J Bacteriol. 1999 Sep;181(17):5355-64. doi: 10.1128/JB.181.17.5355-5364.1999.
10
Parallel molecular genetic analysis.平行分子遗传学分析
Eur J Hum Genet. 1998 Sep-Oct;6(5):417-29. doi: 10.1038/sj.ejhg.5200218.
Zotero 插件