Echavarria Marcela, Sanchez Jose L, Kolavic-Gray Shellie A, Polyak Christina S, Mitchell-Raymundo Felicia, Innis Bruce L, Vaughn David, Reynolds Richard, Binn Leonard N
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
J Clin Microbiol. 2003 Feb;41(2):810-2. doi: 10.1128/JCM.41.2.810-812.2003.
We evaluated the performance of a generic PCR test to detect adenoviruses (AdV) in throat swab specimens collected from asymptomatic and ill military recruits with acute respiratory disease. Samples (n = 210) were collected at entry to basic training and at the time of large outbreaks of AdV-associated acute respiratory disease among military recruits at Fort Jackson, South Carolina, from 1997 to 1998. Compared to cell culture, a sensitivity of 99% and a specificity of 98% were noted for the PCR method to detect AdV in throat swabs. Similar results were obtained with or without DNA extraction, suggesting the absence of significant inhibitors for the PCR method in throat swab samples. No AdV was detected by culture or PCR in throat swabs from healthy recruits, suggesting the absence of latency or asymptomatic shedding. Throat swab specimens proved to be adequate, noninvasive samples to rapidly diagnose respiratory disease in young adults. This generic direct PCR proved to be a useful test for the rapid diagnosis of AdV-associated respiratory disease, detecting all serotypes tested to date and furnishing results within 6 h of specimen arrival. The use of this direct, rapid, sensitive, and specific assay would assist health care providers and public health practitioners in the early diagnosis, management, and control of AdV-associated respiratory disease.
我们评估了一种通用聚合酶链反应（PCR）检测方法在检测从无症状及患病的患有急性呼吸道疾病的新兵咽喉拭子标本中腺病毒（AdV）的性能。样本（n = 210）于1997年至1998年在南卡罗来纳州杰克逊堡新兵进入基础训练时以及新兵中腺病毒相关急性呼吸道疾病大规模爆发时采集。与细胞培养相比，PCR方法检测咽喉拭子中腺病毒的灵敏度为99%，特异性为98%。无论有无DNA提取，均获得了相似结果，这表明咽喉拭子样本中不存在对PCR方法有显著抑制作用的物质。在健康新兵的咽喉拭子中，通过培养或PCR均未检测到腺病毒，这表明不存在潜伏感染或无症状排毒情况。咽喉拭子标本被证明是用于快速诊断年轻成年人呼吸道疾病的合适、非侵入性样本。这种通用的直接PCR被证明是一种用于快速诊断腺病毒相关呼吸道疾病的有用检测方法，能检测迄今测试的所有血清型，并在标本送达后6小时内提供结果。使用这种直接、快速、灵敏且特异的检测方法将有助于医护人员和公共卫生从业者对腺病毒相关呼吸道疾病进行早期诊断、管理和控制。
